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標題: | 非接觸式密度與濃度測量裝置的設計 Design of non-contact density and concentration measuring device |
作者: | 蕭聖洋 Sheng-Yang Xiao |
指導教授: | 邵耀華 Yio-Wha Shau |
關鍵字: | 折射率量測,管流量測,動態量測,逆流離心淘析,細胞收穫系統, Refractive index measurement,tube flow measurement,dynamic measurement,counterflow centrifugal elutriation,cell harvesting system, |
出版年 : | 2023 |
學位: | 碩士 |
摘要: | 本研究主要分為離心清洗系統與量測系統兩大部分。量測系統使用雷射作為光源,並使用光敏電阻作為光接收器,利用稜鏡分光特性和光學原理的應用,透過Snell’s law與Beer’s law的轉換,將電訊號同時轉換為具有物理意義的濃度與密度量測值,提供非接觸式量測動態管流的功能。同時,使用自製的逆流離心系統,將培養基替代品之8%鹽水與細胞替代品PS聚苯乙烯塑膠微粒混合,模擬細胞收穫流程進行分離與清洗。最後將兩者結合,使用量測系統來動態監測鹽水稀釋濃度。研究結果顯示本系統的動態濃度量測結果最大誤差為15%,但在稀釋過程中會逐漸減小。當稀釋濃度降為目標濃度時,誤差為3.1%。使用圓管量測折射率時,根據入射角的不同產生的光學現象,折射率最大誤差為13.5%。但最終本系統量測動態鹽水稀釋密度的最大誤差僅為1.9%。
以上結果驗證了本研究之量測系統在細胞收穫系統中的可行性,提供了一種可以應用在細胞收穫程序的非接觸式量測管流濃度與密度的系統。 The present study is divided into two main parts: the centrifugal cleaning system and the measurement system. The measurement system utilizes a laser as the light source and employs a photoresistor as the light receiver. By applying the principles of prism dispersion and optical theory, and through the transformation based on Snell's law and Beer's law, the electrical signals are converted into physically meaningful concentration and density measurements. This system provides a non-contact method for dynamically measuring fluid flow in tubes. Additionally, a self-made counterflow centrifugation system is utilized to separate and clean a mixture of 8% saline solution (as a substitute for culture medium) and PS polystyrene plastic particles (as a substitute for cells), simulating the cell harvesting process. Finally, both systems are combined, and the measurement system is used to dynamically monitor the dilution concentration of the saline solution. The research results showed that the maximum error in the dynamic concentration measurement of this system is 15%. However, it gradually decreases during the dilution process. When the dilution concentration reaches the target concentration, the error is reduced to 3.1%. When measuring the refractive index using a cylindrical tube, the maximum error due to different incident angles and resulting optical phenomena is 13.5%. However, in the end, the maximum error in measuring the dynamic saline dilution density with this system is only 1.9%. These results verify the feasibility of the measurement system in the context of cell harvesting systems, providing a non-contact measurement system for monitoring flow concentration and density that can be applied in cell harvesting procedures. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/88459 |
DOI: | 10.6342/NTU202302345 |
全文授權: | 同意授權(限校園內公開) |
顯示於系所單位: | 應用力學研究所 |
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