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標題: | 具有抗病毒以及抗菌特性的硫酸化多醣-殼聚醣奈米複合粒子作為藥物載體的研究 Antiviral and antibacterial sulfated polysaccharide-chitosan nanocomposite particles as drug carrier |
作者: | 殷愛宜 Ai-Yi Yin |
指導教授: | 徐善慧 Shan-hui Hsu |
關鍵字: | 節旋藻,硫酸化多醣,奈米粒子,抗菌,抗病毒,藥物輸送, Arthrospira,Sulfated polysaccharide,Nanoparticles,Antibacterial,Antiviral,Drug Delivery, |
出版年 : | 2023 |
學位: | 碩士 |
摘要: | 藥物輸送系統 (DDS) 是將藥物輸送到目標部位且風險最小的方法。而其中有一種具有潛力的策略是利用聚合物奈米粒子作為藥物載體,使用的聚合物需具備生物相容性和可降解的特性,以降低對於人體的傷害。在本篇研究,開發了由節旋萃取硫酸化多醣和殼聚醣組成的奈米粒子,並期望透過兩種材料的合成,結合兩者的優勢。得到具有抗病毒、抗菌和pH敏感的多功能複合奈米粒子,縮寫為APC。在接近人體的生理環境中 (pH = 7.4),透過不同的比例針對APC奈米粒子的形態和尺寸 (~160 nm)進行穩定性的觀察並進行優化。優化過的APC奈米粒子在體外驗證了有效的抗菌(有效濃度2μg/mL)和抗病毒(有效濃度6.596 μg/mL)特性。 並且在不同pH值下的環境,針對各種類別的藥物(包括親水性藥物、疏水性藥物和蛋白質藥物)檢驗了APC的載藥可行性, 針對pH敏感的藥物釋放行為和藥物動力學。也在肺癌細胞和神經幹細胞中評估了應用。使用APC作為載藥奈米粒子,能夠保持藥物的生物活性,以抑制肺癌細胞的增殖(減少約 40%),同時能夠減輕藥物對神經幹細胞的抑制作用。這些發現表明,硫酸化多醣-殼聚醣的生物相容性複合奈米粒子很好地保持了抗病毒和抗菌特性並具有pH敏感的藥物釋放作用,可作為一種有前途的多功能藥物載體用於進一步的生物醫學應用。 Drug delivery systems (DDS) are methods of delivering drugs to targeted sites with minimal risk. One popular strategy of DDS is using nanoparticles as a drug carrier, which are made from biocompatible and degradable polymers. Herein, nanoparticles composed of Arthrospira-derived sulfated polysaccharide (AP) and chitosan were developed and expected to possess the capabilities of antiviral, antibacterial, and pH-sensitive properties. The composite nanoparticles, abbreviated as APC, were optimized for stability of morphology and size (~160 nm) in the physiological environment (pH=7.4). Potent antibacterial (over 2 μg/mL) and antiviral (over 6.596 μg/mL) properties were verified in vitro. The pH-sensitive release behavior and release kinetics of drug-loaded APC nanoparticles were examined for various categories of drugs, including hydrophilic, hydrophobic, and protein drugs, under different pH values of the surroundings. Effects of APC nanoparticles were also evaluated in lung cancer cells and neural stem cells. The use of APC nanoparticles as a drug delivery system maintained the bioactivity of drug to inhibit the proliferation of lung cancer cells (with ~40% reduction) and to relieve the growth inhibitory effect on neural stem cells. These findings indicate that the pH-sensitive and biocompatible composite nanoparticles of sulfated polysaccharide and chitosan well keep the antiviral and antibacterial properties and may serve as a promising multifunctional drug carrier for further biomedical applications. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/87786 |
DOI: | 10.6342/NTU202300717 |
全文授權: | 同意授權(全球公開) |
顯示於系所單位: | 高分子科學與工程學研究所 |
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