纖維素奈米纖維之益生質功效研究

Abstract

近幾年，益生菌及益生質被廣泛的探討及研究。纖維素為一種膳食纖維，無法在胃腸道中消化，其在應用上具有一些缺點（如高吸水性），為了克服這些缺點而生產出纖維素奈米纖維（cellulose nanofiber，CNF），使其除了保有纖維素的特性外，也具有更好的應用性。 本研究目的為評估纖維素奈米纖維之益生質特性，將纖維素奈米纖維預處理後進行物化及益生質特性分析。益生質分析中使用的菌株為Lactobacillus acidophilus BCRC 14079及Bifidobacterium longum BCRC 14602作為目標菌株，觀察兩株菌於不同碳源配方的培養基中之生長存活能力。正控制組以已知的益生質（果寡糖）作為碳源添加於培養基。結果顯示，經高壓處理之長製程纖維素奈米纖維與果寡糖組別在L. acidophilus的活菌數具有相同的結果，而另一株菌B. longum中無論在活菌數或pH值果寡糖皆有最佳效果，且纖維素奈米纖維組別顯著高於未額外添加碳源的組別。由結果得知，纖維素奈米纖維具有益生質特性，但不同製備時間、加工處理及不同菌株皆會影響其益生質特性，因此需進行更多探討。本實驗結果證實纖維素奈米纖維有望成為新興之益生質物質，可增加其於食品領域中的應用性。

In the recent years, probiotics and prebiotics have been extensively researched. Cellulose is a dietary fiber, as a non-digestible food ingredients, has some disadvantages in application (e.g. high water absorption). In order to overcome these, cellulose nanofibers (CNF) weree produced, maintaining the characteristics of cellulose and having better applicability. The purpose of this study was to evaluate the prebiotic properties of cellulose nanofibers. After pretreatment of cellulose nanofibers, we analyzed the physicochemical and the prebiotic properties of it. In prebiotic analysis: Lactobacillus acidophilus BCRC 14079 and Bifidobacterium longum BCRC 14602 as the target strains, observed the growth and survival ability of the two strains in different carbon source medium. The positive control group was added to the culture medium with known prebiotic (fructose oligosaccharides, FOS) as a carbon source. The results showed that the long production CNF treated with high pressure has the same results as FOS in L. acidophilus viable count, and the results of another strain B. longum showed that FOS has the best effect in viable count and pH value. However, the CNF group was significantly higher than the group without additional carbon source. In the results, we found that CNF has the prebiotic properties, but the different production flow, processing treatments and different strains will affect their prebiotic properties. So more discussion is needed. The results of this experiment confirm that cellulose nanofiber is expected to become an emerging probiotic substance, which can increase its applicability in the food field.