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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 丁俞文(Yu-Wen Ting) | |
| dc.contributor.author | Chun-Wei Wu | en |
| dc.contributor.author | 吳峻瑋 | zh_TW |
| dc.date.accessioned | 2021-07-11T14:53:12Z | - |
| dc.date.available | 2025-07-29 | |
| dc.date.copyright | 2020-07-31 | |
| dc.date.issued | 2020 | |
| dc.date.submitted | 2020-07-23 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78364 | - |
| dc.description.abstract | 近幾年,益生菌及益生質被廣泛的探討及研究。纖維素為一種膳食纖維,無法在胃腸道中消化,其在應用上具有一些缺點(如高吸水性),為了克服這些缺點而生產出纖維素奈米纖維(cellulose nanofiber,CNF),使其除了保有纖維素的特性外,也具有更好的應用性。 本研究目的為評估纖維素奈米纖維之益生質特性,將纖維素奈米纖維預處理後進行物化及益生質特性分析。益生質分析中使用的菌株為Lactobacillus acidophilus BCRC 14079及Bifidobacterium longum BCRC 14602作為目標菌株,觀察兩株菌於不同碳源配方的培養基中之生長存活能力。正控制組以已知的益生質(果寡糖)作為碳源添加於培養基。結果顯示,經高壓處理之長製程纖維素奈米纖維與果寡糖組別在L. acidophilus的活菌數具有相同的結果,而另一株菌B. longum中無論在活菌數或pH值果寡糖皆有最佳效果,且纖維素奈米纖維組別顯著高於未額外添加碳源的組別。由結果得知,纖維素奈米纖維具有益生質特性,但不同製備時間、加工處理及不同菌株皆會影響其益生質特性,因此需進行更多探討。本實驗結果證實纖維素奈米纖維有望成為新興之益生質物質,可增加其於食品領域中的應用性。 | zh_TW |
| dc.description.abstract | In the recent years, probiotics and prebiotics have been extensively researched. Cellulose is a dietary fiber, as a non-digestible food ingredients, has some disadvantages in application (e.g. high water absorption). In order to overcome these, cellulose nanofibers (CNF) weree produced, maintaining the characteristics of cellulose and having better applicability. The purpose of this study was to evaluate the prebiotic properties of cellulose nanofibers. After pretreatment of cellulose nanofibers, we analyzed the physicochemical and the prebiotic properties of it. In prebiotic analysis: Lactobacillus acidophilus BCRC 14079 and Bifidobacterium longum BCRC 14602 as the target strains, observed the growth and survival ability of the two strains in different carbon source medium. The positive control group was added to the culture medium with known prebiotic (fructose oligosaccharides, FOS) as a carbon source. The results showed that the long production CNF treated with high pressure has the same results as FOS in L. acidophilus viable count, and the results of another strain B. longum showed that FOS has the best effect in viable count and pH value. However, the CNF group was significantly higher than the group without additional carbon source. In the results, we found that CNF has the prebiotic properties, but the different production flow, processing treatments and different strains will affect their prebiotic properties. So more discussion is needed. The results of this experiment confirm that cellulose nanofiber is expected to become an emerging probiotic substance, which can increase its applicability in the food field. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-11T14:53:12Z (GMT). No. of bitstreams: 1 U0001-2207202022305300.pdf: 3910673 bytes, checksum: efa08fc09615ca66b8268f38547af132 (MD5) Previous issue date: 2020 | en |
| dc.description.tableofcontents | 致謝 I 摘要 III ABSTRACT IV 目錄 VI 圖目錄 IX 表目錄 X 第一章、前言 1 第二章、文獻回顧 3 2.1 益生菌 3 2.1.1益生菌概述及定義 3 2.1.2乳酸菌 4 2.1.3益生菌健康生理功效 6 2.1.4益生菌的控管 11 2.2 益生質 12 2.2.1益生質概述及定義 12 2.2.2益生質分類 16 2.2.3益生質健康益處 18 2.2.4益生質應用及未來趨勢 21 2.3纖維素 23 2.3.1纖維素概述 23 2.3.2結構組成 24 2.3.3纖維素特性 25 2.3.4纖維素應用 26 2.4奈米纖維 27 2.4.1奈米纖維概述 27 2.4.2奈米纖維定義 27 2.4.3奈米纖維生產方式 29 2.4.4奈米纖維種類 30 2.4.5奈米纖維應用 33 第三章、研究目的與實驗架構 35 3.1研究目的 35 3.2實驗架構 35 第四章、材料與方法 36 4.1實驗材料 36 4.1.1藥品 36 4.1.2實驗原料 37 4.1.2實驗菌株 37 4.1.3實驗儀器設備 38 4.2實驗方法 39 4.2.1 奈米纖維素預處理 39 4.2.2 奈米纖維素酸處理之糖含量變化 39 4.2.3奈米纖維素溶解度 40 4.2.4奈米纖維素型態結構觀察 40 4.2.5 菌種保存與活化 40 4.2.6 培養基製備 41 4.2.7 不同碳源培養基 41 4.2.8 奈米纖維素培養基製備 42 4.2.9 初始菌數檢測 42 4.2.10 益生菌生長曲線 42 4.2.11益生菌活菌數量 43 4.2.12 培養液pH值及可滴定酸度 43 4.2.13 培養液糖含量 44 4.2.14 統計分析與圖表繪製 44 第五章、結果與討論 45 5.1奈米纖維素預處理 45 5.2奈米纖維素糖含量變化 45 5.3奈米纖維素溶解度 46 5.4奈米纖維素型態結構觀察 49 5.5初始菌數檢測 51 5.6益生菌生長曲線 52 5.7益生菌活菌數量 55 5.8培養液PH值及可滴定酸度 57 5.9培養液糖含量 61 第六章、結論 63 第七章、未來展望 64 第八章、參考文獻 65 附錄1 ⅰ 附錄2 ⅱ | |
| dc.language.iso | zh-TW | |
| dc.subject | 膳食纖維 | zh_TW |
| dc.subject | 胃腸道微生物 | zh_TW |
| dc.subject | 益生菌 | zh_TW |
| dc.subject | 益生質 | zh_TW |
| dc.subject | 纖維素奈米纖維 | zh_TW |
| dc.subject | Prebiotic | en |
| dc.subject | Gastrointestinal microorganism | en |
| dc.subject | Cellulose nanofiber | en |
| dc.subject | Dietary fiber | en |
| dc.subject | Probiotic | en |
| dc.title | 纖維素奈米纖維之益生質功效研究 | zh_TW |
| dc.title | Investigation of the Prebiotic Effects of Cellulose Nanofibers | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 108-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 吳瑞碧(Swi-Bea Wu),沈賜川(Szu-Chuan Shen),鄭光成(Kuan-Chen Cheng),蔡宗佑(Tsung-Yu Tsai) | |
| dc.subject.keyword | 胃腸道微生物,益生菌,益生質,膳食纖維,纖維素奈米纖維, | zh_TW |
| dc.subject.keyword | Gastrointestinal microorganism,Probiotic,Prebiotic,Dietary fiber,Cellulose nanofiber, | en |
| dc.relation.page | 103 | |
| dc.identifier.doi | 10.6342/NTU202001756 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2020-07-24 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 食品科技研究所 | zh_TW |
| dc.date.embargo-lift | 2025-07-29 | - |
| 顯示於系所單位: | 食品科技研究所 | |
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