次世代基因定序於於藻類上的應用與生理分析

Abstract

全球暖化造成人類的生存危機，二氧化碳的大量排放也對氣候造成影響。為因應溫室效應的威脅，減少對石化能源的依賴，人類不停地思考各種不同的替代能源。因為微藻具有多樣性，而且是地球上生長最快的植物，所以被認為是捕獲二氧化碳的最有效和最環保的物種。第二代基因定序，也稱為次世代基因測序，始於2005年左右。在不同的時期，它提供了不同的技術和平台。藻類雖然是生質能源的料源之一，有些品種也以生產高單價健康食品聞名，但在研究上因為藻類基因資訊的缺乏，使得進展有限。在次世代基因定序開始發展之後，本論文便以次世代基因定序中的轉錄體開始，分析不同條件下藻類的生長。

Global warming causes the crisis of human survival, and amount of carbon dioxide emission has a significant impact on the climate. In response to the threat of the greenhouse effect and reduce the dependence on the petrochemical energy, several kinds of alternative energy sources were considered by human. Microalgae are considered to be the most effective and environmentally friendly species to capture carbon dioxide among them because microalgae has ecological diversity can grow under different stress and it is the fastest growing plants on the earth. The second-generation sequencing technologies, also called next-generation sequencing, began around 2005. During different period of time, it provides different technology and platforms. Algae are one of the sources of biomass energy. Some species are also known for producing high-priced healthy foods. However, the algae research process is limited because lacking of algae genetic information. Hence, the research developments of the next generation sequencing analysis methods with transcriptome, and studies the algae growth under different conditions.