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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳仁治(Jen-Chih Chen) | |
| dc.contributor.author | Tze-Jung Yeh | en |
| dc.contributor.author | 葉慈容 | zh_TW |
| dc.date.accessioned | 2021-07-11T14:36:20Z | - |
| dc.date.available | 2022-08-31 | |
| dc.date.copyright | 2017-08-31 | |
| dc.date.issued | 2017 | |
| dc.date.submitted | 2017-08-17 | |
| dc.identifier.citation | 1. Lopez, C.V.G., et al., Utilization of the cyanobacteria Anabaena sp ATCC 33047 in CO2 removal processes. Bioresource Technology, 2009. 100(23): p. 5904-5910.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/77868 | - |
| dc.description.abstract | 全球暖化造成人類的生存危機，二氧化碳的大量排放也對氣候造成影響。為因應溫室效應的威脅，減少對石化能源的依賴，人類不停地思考各種不同的替代能源。因為微藻具有多樣性，而且是地球上生長最快的植物，所以被認為是捕獲二氧化碳的最有效和最環保的物種。第二代基因定序，也稱為次世代基因測序，始於2005年左右。在不同的時期，它提供了不同的技術和平台。藻類雖然是生質能源的料源之一，有些品種也以生產高單價健康食品聞名，但在研究上因為藻類基因資訊的缺乏，使得進展有限。在次世代基因定序開始發展之後，本論文便以次世代基因定序中的轉錄體開始，分析不同條件下藻類的生長。 | zh_TW |
| dc.description.abstract | Global warming causes the crisis of human survival, and amount of carbon dioxide emission has a significant impact on the climate. In response to the threat of the greenhouse effect and reduce the dependence on the petrochemical energy, several kinds of alternative energy sources were considered by human. Microalgae are considered to be the most effective and environmentally friendly species to capture carbon dioxide among them because microalgae has ecological diversity can grow under different stress and it is the fastest growing plants on the earth. The second-generation sequencing technologies, also called next-generation sequencing, began around 2005. During different period of time, it provides different technology and platforms. Algae are one of the sources of biomass energy. Some species are also known for producing high-priced healthy foods. However, the algae research process is limited because lacking of algae genetic information. Hence, the research developments of the next generation sequencing analysis methods with transcriptome, and studies the algae growth under different conditions. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-11T14:36:20Z (GMT). No. of bitstreams: 1 ntu-106-D96642008-1.pdf: 3234944 bytes, checksum: fdf00e72bff13720f617c46d9b2316db (MD5) Previous issue date: 2017 | en |
| dc.description.tableofcontents | Chapter 1 The applications of RNA-seq techniques in microalgae 1
1.1 Algae can produce high-value products 1 1.2 RNA-seq History 2 1.3 The applications of RNA-seq techniques in microalgae 3 Chapter 2 Transcriptome and physiological analysis of a lutein-producing alga Desmodesmus sp. reveals the molecular mechanisms for high lutein productivity 5 2.1 Introduction 7 2.2 Material and methods 10 2.2.1 Microalgal culture and treatment 10 2.2.2 Species identification and phylogenetic analysis 11 2.2.3 Determination of cell growth 12 2.2.4 Determination of residual nitrate concentration in the medium 12 2.2.5 RNA extraction and high-throughput sequencing 13 2.2.6 de novo transcriptome assembly 13 2.2.7 Functional annotation of Desmodesmus sp. JSC3 transcriptome 15 2.2.8 Expression analysis 16 2.2.9 Gene Ontology (GO) analysis 16 2.2.10 Quantitative real-time PCR (qPCR) 16 2.2.11 Determination of photosynthetic O2 evolution rate and respiration rate 17 2.2.12 Spectrophotometric determination of carotenoids and chlorophyll contents 18 2.2.13 Chromatographic analysis of lutein and other carotenoids 18 2.2.14 Chlorophyll a fluorescence determination of PSII activity using pulse amplitude modulation (PAM) fluorometry 19 2.2.15 Determination of nitrate concentration in the medium and total carbon and total nitrogen 20 2.2.16 Statistics 20 2.3 Results 21 2.3.1 Identification of a unicellular microalga accumulating high level of lutein after a period of culture as Desmodesmus sp. JSC3 21 2.3.2 de novo assembly of the Desmodesmus sp. JSC3 transcriptome and unigene annotation 27 2.3.3 GO enrichment analysis suggests that photosynthesis and pigment biosynthesis underwent extensive changes when Desmodesmus sp. JSC3 accumulated lutein 28 2.3.4 Analysis of the Desmodesmus sp. JSC3 transcriptome for genes involved in lutein biosynthesis 32 2.3.5 Transcriptome analysis of genes responsible for enhanced chlorophyll biosynthesis 35 2.3.6 Changes in photosynthetic activity and its associated transcriptome during lutein accumulation in a batch culture 40 2.4 Discussion 47 2.5 Conclusion 56 Chapter 3 Transcriptome analysis of nitrogen prolong culture induced carbohydrate accumulation in Desmodesmus sp. CNW-1 57 3.1 Introduction 58 3.2 Material and methods 59 3.2.1 Microalgal culture and treatment 59 3.2.2 Species identification and phylogenetic analysis 60 3.2.3 Determination of cell growth 61 3.2.4 RNA extraction and high-throughput sequencing 61 3.2.5 De novo transcriptome assembly 62 3.2.6 Functional annotation of Desmodesmus sp. CNW-1 transcriptome 63 3.2.7 Expression analysis and Gene Ontology (GO) analysis 64 3.3 Results 65 3.3.1 Identification of Desmodesmus sp. CNW–1 65 3.3.2 CNW physiological results 66 3.3.3 De novo assembly of the Desmodesmus sp. CNW–N transcriptome and unigene annotation 67 3.3.4 CNW-1 GO enrichment analysis 69 3.3.5 Analysis of the Desmodesmus sp. CNW-1 transcriptome for genes involved in starch biosynthesis 73 3.3.6 Transcriptome analysis of genes responsible for nitrogen starvation 76 3.4 Discussions 78 Chapter 4 Conclusions 79 REFERENCE 80 | |
| dc.language.iso | en | |
| dc.title | 次世代基因定序於於藻類上的應用與生理分析 | zh_TW |
| dc.title | Application of next-generation sequencing and physiological analyses in microalgae | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 105-2 | |
| dc.description.degree | 博士 | |
| dc.contributor.oralexamcommittee | 高成炎(Cheng-Yan Kao),李澤民(Tse-Min Lee),張嘉修(Jo-Shu Chang),朱學亭(Hsueh-Ting Chu) | |
| dc.subject.keyword | 微藻,次世代基因測序,轉錄體,生質能源,健康食品, | zh_TW |
| dc.subject.keyword | microalgae,next-generation sequencing,transcriptome,alternative energy,healthy foods, | en |
| dc.relation.page | 85 | |
| dc.identifier.doi | 10.6342/NTU201703631 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2017-08-18 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 生物科技研究所 | zh_TW |
| 顯示於系所單位： | 生物科技研究所 | |
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