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標題: | 小鼠巨噬細胞RAW264.7中hnRNP K磷酸化對COX-2 mRNA穩定度的調控 The regulatory role of hnRNP K phosphorylation to COX-2 mRNA stability in mouse macrophage RAW264.7 cells |
作者: | Pei-Yu Chiang 江佩鈺 |
指導教授: | 張?仁(Ching-Jin Chang,) |
關鍵字: | 脂多醣、巨噬細胞、RAW264.7 cell、異質性核核糖核蛋白K、環氧化酶,2、轉錄後調控。, LPS, macrophage, RAW264.7, hnRNP K, COX-2, post-transcriptional regulation, |
出版年 : | 2012 |
學位: | 博士 |
摘要: | 當病原體感染發生在生物體中,許多細胞因子或即早基因,應迅速,準確地打開和關閉以進行免疫反應。嚴格地控制這些基因表現對預防免疫系統疾病是非常重要的。而利用RNA結合蛋白參與的轉錄後調控則是細胞用來操縱基因表達的策略之一。異質性核核糖核蛋白K,擁有三個可與RNA結合的KH(K-homology)結構域,並常涉及在調控後轉錄和轉譯的過程。為了研究hnRNP K在免疫反應中可能的功能,在脂多醣(LPS)處理過的小鼠RAW264.7巨噬細胞萃取物中以hnRNP K抗體進行RNA免疫沉澱。兩個即早基因:Tistetraproline(TTP)和腫瘤壞死因子- When pathogen infection occurrs in organism, many cytokines or immediate genes (IEGs) are rapidly and precisely expressed and/or repressed for performing immune responses. Tight control of these genes is very important for preventing immune disorders. Post-transcriptional regulation involving in trans-acting RNA-binding proteins is one of the favorite strategies of cells to manipulate gene expression. Heterogeneous nuclear ribonucleoprotein K (hnRNP K), containing three KH (K-homology) domains that mediate RNA binding, is involved in the regulation of post-transcription and translation processes. To study the possible functions of hnRNP K in immune response, I performed anti-hnRNP K RNA immunoprecipitation assay with cell extracts from lipopolysaccharide (LPS)-treated mouse RAW264.7 macrophages. Two IEGs, Tistetraproline (TTP) and Tumor necrosis factor |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/65594 |
全文授權: | 有償授權 |
顯示於系所單位: | 生化科學研究所 |
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