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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 張?仁(Ching-Jin Chang,) | |
dc.contributor.author | Pei-Yu Chiang | en |
dc.contributor.author | 江佩鈺 | zh_TW |
dc.date.accessioned | 2021-06-16T23:52:43Z | - |
dc.date.available | 2012-07-27 | |
dc.date.copyright | 2012-07-27 | |
dc.date.issued | 2012 | |
dc.date.submitted | 2012-07-19 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/65594 | - |
dc.description.abstract | 當病原體感染發生在生物體中,許多細胞因子或即早基因,應迅速,準確地打開和關閉以進行免疫反應。嚴格地控制這些基因表現對預防免疫系統疾病是非常重要的。而利用RNA結合蛋白參與的轉錄後調控則是細胞用來操縱基因表達的策略之一。異質性核核糖核蛋白K,擁有三個可與RNA結合的KH(K-homology)結構域,並常涉及在調控後轉錄和轉譯的過程。為了研究hnRNP K在免疫反應中可能的功能,在脂多醣(LPS)處理過的小鼠RAW264.7巨噬細胞萃取物中以hnRNP K抗體進行RNA免疫沉澱。兩個即早基因:Tistetraproline(TTP)和腫瘤壞死因子- | zh_TW |
dc.description.abstract | When pathogen infection occurrs in organism, many cytokines or immediate genes (IEGs) are rapidly and precisely expressed and/or repressed for performing immune responses. Tight control of these genes is very important for preventing immune disorders. Post-transcriptional regulation involving in trans-acting RNA-binding proteins is one of the favorite strategies of cells to manipulate gene expression. Heterogeneous nuclear ribonucleoprotein K (hnRNP K), containing three KH (K-homology) domains that mediate RNA binding, is involved in the regulation of post-transcription and translation processes. To study the possible functions of hnRNP K in immune response, I performed anti-hnRNP K RNA immunoprecipitation assay with cell extracts from lipopolysaccharide (LPS)-treated mouse RAW264.7 macrophages. Two IEGs, Tistetraproline (TTP) and Tumor necrosis factor | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T23:52:43Z (GMT). No. of bitstreams: 1 ntu-101-D94b46007-1.pdf: 2995327 bytes, checksum: d4f707b64c59947ce1300ae5490c759b (MD5) Previous issue date: 2012 | en |
dc.description.tableofcontents | 口試委員會審定書 i
誌謝 ii 中文摘要 iv ABSTRACT vi CONTENTS viii LIST OF FIGURES xi LIST OF TABLES xii ABBREVIATIONS xiii INTRODUCTION 1 1. hnRNP K 1 2. The post-translational modification of hnRNP K 4 3. COX-2 6 4. Processing body (P-body) 10 MATERIAS AND METHODS 12 1. Plasmid Constructs 12 2. Cell Culture and Transfection 12 3. Cell Extract Preparation 13 4. Determination of mRNA half-life 13 5. RNA extraction and reverse transcription 14 6. Real-time PCR 14 7. SDS-PAGE and Western Blotting Analysis 15 8. RNA-immunoprecipitation (RNA-IP) 15 9. RNA pull-down assay 16 10. Dual-Luciferase reporter assay 16 11. shRNA 17 12. Lentiviral knockdown 17 13. Expression and Purification of Recombinant Protein 18 14. In Vitro Phosphorylation Assay 18 15. Coimmunoprecipitation (Co-IP) Assays 18 16. Indirect Immunofluorescence and Confocal Microscopy 19 17. Rabbit anti-Phospho-hnRNP K/Ser116 and Ser284 Antibody Generation 19 RESULTS 20 LPS induces COX-2 expression and modulates COX-2 mRNA stability in RAW264.7 cells. 20 COX-2 mRNA is identified as one of the hnRNP K binding targets in LPS activated RAW264.7 cells. 20 hnRNP K downregulated COX-2 3’UTR-containing luciferase activity. 21 hnRNP K plays distinct roles in modulating COX-2 mRNA stability during LPS-stimulation. 22 LPS-induced ERK-signaling phosphorylates Ser116 on hnRNP K 23 Phosphorylation of Ser116 on hnRNP K arises in cytoplasm. 25 Phosphorylation of Ser116 on hnRNP K regulates COX-2 mRNA stability. 25 Phosphorylation of Ser116 is required for cytoplasmic localization of hnRNP K 26 hnRNP K interacts with DDX6, HuR and TTP 27 DDX6 is involved in the Ser116-mediated downregulation of COX-2 expression. 28 DISCUSSION 30 FIGURES 37 TABLE 64 REFERENCE 66 | |
dc.language.iso | en | |
dc.title | 小鼠巨噬細胞RAW264.7中hnRNP K磷酸化對COX-2 mRNA穩定度的調控 | zh_TW |
dc.title | The regulatory role of hnRNP K phosphorylation to COX-2 mRNA stability in mouse macrophage RAW264.7 cells | en |
dc.type | Thesis | |
dc.date.schoolyear | 100-2 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 呂勝春,朱善德,李玉梅,譚賢明 | |
dc.subject.keyword | 脂多醣、巨噬細胞、RAW264.7 cell、異質性核核糖核蛋白K、環氧化酶,2、轉錄後調控。, | zh_TW |
dc.subject.keyword | LPS, macrophage, RAW264.7, hnRNP K, COX-2, post-transcriptional regulation, | en |
dc.relation.page | 84 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2012-07-20 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科學研究所 | zh_TW |
顯示於系所單位: | 生化科學研究所 |
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