施加電刺激以促進神經分化之高孔洞導電支架

Abstract

隨著組織工程的發展，許多研究致力於合成更具生物相似性的生醫材料以模擬人體中的環境，而具有孔洞結構的三維生物支架便是其中一種，神經系統是人體不可或缺的構造之一，然而，因為意外造成的斷裂往往造成神經系統難以修復，許多研究顯示，導電高分子PEDOT的支架擁有良好的生物相容性與導電性，利用PEDOT所製成的孔洞支架在引導神經生長的方面具有非常好的潛力，然而在許多以PEDOT支架進行神經培養的研究中，同時結合電刺激的神經分化成果並不多。在我們的研究中，我們製備了混合了不同濃度奈米碳管（MWCNTs）的PEDOT:PSS導電多孔支架，奈米碳管不僅增加支架的機械強度也提升了導電度，此外，我們將PC12神經細胞培養於立體裝置中並在培養過程中施予電刺激，在電刺激的促使之下，培養於PEDOT:PSS/MWCNT的神經細胞有分化為更成熟的形態的成果。

Three dimensional porous scaffold has been considered as a more appropriate platform for tissue engineering as it better mimic the topological environment if well designed. Nerve tissue can fail to self-regenerate due to the separation of neurons. PEDOT-based conductive scaffold has shown its great performance in biocompatibility and electrical conductivity. Much research has been done on the PEDOT-based scaffolds as a promising guidance channel for neuroregeneration. However, neural culture combined with electrical stimulation was missed. In this study, PEDOT:PSS/MWCNT scaffolds was fabricated with enhanced mechanical properties and electrical conductivity. Also we display a 3D assembled device for electrical-stimulated neural cell culture. Our scaffolds support cell attachment and migration. In addition, PC12 cultured on MWCNT doped scaffolds was induced to differentiate toward more mature neuronal phenotype with the treatment of electrical stimulation.