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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/58439完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 游佳欣(Jiashing Yu) | |
| dc.contributor.author | I-Hsiang Liao | en |
| dc.contributor.author | 廖羿翔 | zh_TW |
| dc.date.accessioned | 2021-06-16T08:15:11Z | - |
| dc.date.available | 2021-08-01 | |
| dc.date.copyright | 2020-07-17 | |
| dc.date.issued | 2020 | |
| dc.date.submitted | 2020-07-15 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/58439 | - |
| dc.description.abstract | 隨著組織工程的發展，許多研究致力於合成更具生物相似性的生醫材料以模擬人體中的環境，而具有孔洞結構的三維生物支架便是其中一種，神經系統是人體不可或缺的構造之一，然而，因為意外造成的斷裂往往造成神經系統難以修復，許多研究顯示，導電高分子PEDOT的支架擁有良好的生物相容性與導電性，利用PEDOT所製成的孔洞支架在引導神經生長的方面具有非常好的潛力，然而在許多以PEDOT支架進行神經培養的研究中，同時結合電刺激的神經分化成果並不多。在我們的研究中，我們製備了混合了不同濃度奈米碳管（MWCNTs）的PEDOT:PSS導電多孔支架，奈米碳管不僅增加支架的機械強度也提升了導電度，此外，我們將PC12神經細胞培養於立體裝置中並在培養過程中施予電刺激，在電刺激的促使之下，培養於PEDOT:PSS/MWCNT的神經細胞有分化為更成熟的形態的成果。 | zh_TW |
| dc.description.abstract | Three dimensional porous scaffold has been considered as a more appropriate platform for tissue engineering as it better mimic the topological environment if well designed. Nerve tissue can fail to self-regenerate due to the separation of neurons. PEDOT-based conductive scaffold has shown its great performance in biocompatibility and electrical conductivity. Much research has been done on the PEDOT-based scaffolds as a promising guidance channel for neuroregeneration. However, neural culture combined with electrical stimulation was missed. In this study, PEDOT:PSS/MWCNT scaffolds was fabricated with enhanced mechanical properties and electrical conductivity. Also we display a 3D assembled device for electrical-stimulated neural cell culture. Our scaffolds support cell attachment and migration. In addition, PC12 cultured on MWCNT doped scaffolds was induced to differentiate toward more mature neuronal phenotype with the treatment of electrical stimulation. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T08:15:11Z (GMT). No. of bitstreams: 1 U0001-1307202015494901.pdf: 2341250 bytes, checksum: 7158b3cb2827b2ff5a5948c58da683ce (MD5) Previous issue date: 2020 | en |
| dc.description.tableofcontents | 口試委員審定書 i 致謝 ii 摘要 iii ABSTRACT iv CONTENTS v LIST OF FIGURES viii LIST OF TABLES x Chapter 1 Introduction 1 1.1 Tissue engineering 1 1.1.1 Nerve tissue engineering 1 1.1.2 Factors affecting cell behavior 3 1.1.3 Electrical stimulation on PC12 6 1.2 Three-dimensional scaffold 7 1.2.1 Properties of scaffold materials 7 1.2.2 Conductive polymers 9 1.2.3 Conductivity of PEDOT:PSS 11 1.2.4 Incorporation of carbon nanotubes 14 1.3 Fabrication methods for PEDOT-based porous scaffolds 15 1.3.1 Interfacial polymerization 15 1.3.2 Ice-templating technique 17 1.4 Motivation and aims 19 1.5 Research Framework 19 Chapter 2 Materials and Methods 21 2.1 Chemicals 21 2.2 Instruments 23 2.3 Solution Formula 25 2.4 Methods 27 2.4.1 Preparation of the PEDOT:PSS/MWCNT scaffold 27 2.4.2 Morphological characterization of the scaffold 28 2.4.3 Electrochemical characterization of the scaffold 30 2.4.4 Liquid uptake behavior of the scaffold 31 2.4.5 Compression test 32 2.4.6 Cell culture and seeding 32 2.4.7 Cell culture platform for electrical stimulation 33 2.4.8 Electrical stimulation 36 2.4.9 Biocompatibility analysis 36 2.4.10 In vitro cytotoxicity test 37 2.4.11 Cell adhesion and morphology 38 2.4.12 Immunofluorescence 40 Chapter 3 Results and discussion 42 3.1 Morphological characteristics 42 3.1.1 Microstructure of PEDOT:PSS/MWCNT scaffolds 42 3.1.2 Porosity and pore size distribution 44 3.2 Physical characteristics 47 3.2.1 Liquid uptake behavior 47 3.2.2 Mechanical properties 49 3.2.3 Electrochemical properties 51 3.3 In-vitro experiments 55 3.3.1 Cytotoxicity assay 55 3.3.2 Cell adhesion 57 3.3.3 Cell proliferation 58 3.3.4 Cell migration 60 3.3.5 Cell morphology 63 3.3.6 Gene expression 65 Chapter 4 Conclusions and Future Work 69 Chapter 5 References 71 | |
| dc.language.iso | en | |
| dc.title | 施加電刺激以促進神經分化之高孔洞導電支架 | zh_TW |
| dc.title | Highly Porous Conductive Scaffolds for Electrically-Stimulated Neural Differentiation | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 108-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 羅世強(Shyh-Chyang Luo),蕭育生(Yu-Sheng Hsiao) | |
| dc.subject.keyword | 神經再生,導電高分子,神經支架,孔洞材料,奈米碳管, | zh_TW |
| dc.subject.keyword | Neuroregeneration,conductive polymer,PEDOT,MWCNT,3D scaffold,porous scaffold, | en |
| dc.relation.page | 82 | |
| dc.identifier.doi | 10.6342/NTU202001475 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2020-07-15 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
| 顯示於系所單位： | 化學工程學系 | |
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