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標題: | 探討mesenchymal stem cells對Th2細胞及過敏性疾病的調控 Study on the regulatory role of mesenchymal stem cells on Th2 cells and allergic diseases |
作者: | Yih-Mei Liou 劉羿梅 |
指導教授: | 莊雅惠 |
關鍵字: | 過敏性氣喘,間葉幹細胞,第二型輔助性T細胞, allergic asthma,mesenchymal stem cells (MSCs),Th2 cells, |
出版年 : | 2016 |
學位: | 碩士 |
摘要: | 過敏性氣喘係因第二型輔助性T細胞的調控失調而造成。而間葉幹細胞除了細胞再生的能力之外同時具有免疫調控的能力而被視作一具有潛力的免疫治療。目前將間葉幹細胞應用在由OVA引起的氣喘小鼠動物模式上已有療效,但未有明確證據指出間葉幹細胞是否能直接抑制第二型輔助性T細胞的免疫反應以及間葉幹細胞對過敏性疾病病人的免疫的影響還並不是很清楚。首先我們利用培養的小鼠第二型輔助性T細胞和間葉幹細胞共同培養後發現小鼠第二型輔助性T細胞分泌細胞激素的功能有明顯的降低。接著將間葉幹細胞應用在過敏性氣喘的病患上以探討間葉幹細胞對於過敏疾病的影響。分析從過敏性氣喘病人血液樣本中分離出來的周邊血單核球細胞跟間葉幹細胞共同培養後的結果,發現其中T細胞的增生以及活化都受到抑制,同時由第二型輔助性T細胞分泌的細胞激素也有明顯的減低。而單一阻斷任一由間葉幹細胞產生的免疫抑制相關分子並不影響免疫抑制的結果,因此推斷參與間葉幹細胞進行免疫抑制應同時由多種分子調控。因此我們的實驗證實間葉幹細胞可以減低第二型輔助性T細胞的反應並且抑制過敏性氣喘病人的免疫反應。 Allergic asthma is an airway inflammation mediated by imbalanced type II helper T cell (Th2) immune response. Mesenchymal stem cell (MSC) is nowadays thought to be a potential immunotherapy. MSCs have been shown to suppress allergic asthma in ovalbumin immunized murine model. However, there is no clear evidence that MSCs can suppress Th2 cells directly and the effect of MSCs on allergic disease remains unclear. We cultured mouse Th2 cells to investigate the direct interaction between MSCs and Th2 cells. The result showed MSCs can efficiently suppress the cytokine production ability of Th2 cells. We applied MSCs to patients with allergic asthma to study the effect of MSCs on Th2 related disease. Utilizing peripheral blood mononuclear cells (PBMCs) isolated from asthma patients, we analyzed T cell proliferation and function after cocultured with MSCs and found that MSCs suppressed proliferation and Th2 cytokine production of T cells form asthma patients. This suppression phenotype might depend on multiple factors since blockade of single factor didn’t alter the suppressive effect of MSCs. In conclusion, MSCs is able to suppress the function of Th2 cells and also reduce T cell response in Th2 mediated disease. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/50404 |
DOI: | 10.6342/NTU201601577 |
全文授權: | 有償授權 |
顯示於系所單位: | 醫學檢驗暨生物技術學系 |
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