紅外線照射對PC12發育和基因表現影響及微電極晶片

Abstract

本研究的目的，是想要模擬紅外光照射下老鼠神經細胞的生長情形、蛋白質表現，於是設計一種整合紅外線發射器和二氧化碳培養箱的機器，並使用PC12和加入神經生長因子的PC12作為模擬老鼠神經細胞，來觀察細胞生長情形的表現及蛋白質表現的影響。將PC12和加入神經生長因子的PC12經過波長2um~4.5um之紅外光照射4天後，觀察其細胞覆蓋率和細胞個數以了解生長狀態，再進而使用蛋白質二維電泳分析法分析其表現蛋白質表現，並利用質譜分析出有差異性的蛋白質。實驗結果顯示PC12和加入神經生長因子的PC12發現在紅外光照射下會導致在細胞生長上和蛋白質量的變化。而在紅外光照射1天後，變化最明顯。此外並製作合適的神經細胞晶片，將細胞培養晶片上，來量測細胞電訊號的變化。

The purpose of this study is to simulate the rat neuron cell growth and protein expression in infrared radiation, and design a machine which combine the infrared emitter and carbon dioxide incubator, and utilize the above cells to simulate the rat neuron cells, and to know the cell growth by observing the cell coverage and cell numbers. After 4 days exposure to infrared radiation, the cell coverage and cell numbers of PC12 and PC12 which treated with nerve growth were measured. Two dimensional protein gel electrophoresis and mass spectrometry are also applied to observe the protein expression pattern. From the results, the cell growth and protein quality under infrared radiation are changed. The difference is visible in infrared radiation 1 day. We also design the neuron chip to measure the neuron electric signals.