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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林致廷(Chih-Ting Lin) | |
| dc.contributor.author | Yu-Fu Weng | en |
| dc.contributor.author | 翁裕復 | zh_TW |
| dc.date.accessioned | 2021-06-15T07:05:00Z | - |
| dc.date.available | 2016-01-17 | |
| dc.date.copyright | 2011-01-17 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-12-13 | |
| dc.identifier.citation | 參考文獻
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/48620 | - |
| dc.description.abstract | 本研究的目的，是想要模擬紅外光照射下老鼠神經細胞的生長情形、蛋白質表現，於是設計一種整合紅外線發射器和二氧化碳培養箱的機器，並使用PC12和加入神經生長因子的PC12作為模擬老鼠神經細胞，來觀察細胞生長情形的表現及蛋白質表現的影響。將PC12和加入神經生長因子的PC12經過波長2um~4.5um之紅外光照射4天後，觀察其細胞覆蓋率和細胞個數以了解生長狀態，再進而使用蛋白質二維電泳分析法分析其表現蛋白質表現，並利用質譜分析出有差異性的蛋白質。實驗結果顯示PC12和加入神經生長因子的PC12發現在紅外光照射下會導致在細胞生長上和蛋白質量的變化。而在紅外光照射1天後，變化最明顯。此外並製作合適的神經細胞晶片，將細胞培養晶片上，來量測細胞電訊號的變化。 | zh_TW |
| dc.description.abstract | The purpose of this study is to simulate the rat neuron cell growth and protein expression in infrared radiation, and design a machine which combine the infrared emitter and carbon dioxide incubator, and utilize the above cells to simulate the rat neuron cells, and to know the cell growth by observing the cell coverage and cell numbers. After 4 days exposure to infrared radiation, the cell coverage and cell numbers of PC12 and PC12 which treated with nerve growth were measured. Two dimensional protein gel electrophoresis and mass spectrometry are also applied to observe the protein expression pattern. From the results, the cell growth and protein quality under infrared radiation are changed. The difference is visible in infrared radiation 1 day. We also design the neuron chip to measure the neuron electric signals. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T07:05:00Z (GMT). No. of bitstreams: 1 ntu-99-R97943083-1.pdf: 5156895 bytes, checksum: 3295de839bf6b800b98af74f9e8bed69 (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | 目錄
謝辭 i 摘要 ii Abstract iii 目錄 I 圖目錄 III 第1章 序論 1 1.1 前言 1 1.2 研究動機 3 1.3 論文架構 4 第2章 文獻回顧 5 2.1紅外光與動植物體影響 5 2.2 微電極陣列晶片 7 2.3 結論 9 第3章 實驗原理與流程 10 3.1 簡介 10 3.2 材料 11 3.2.1 老鼠PC12細胞 11 3.2.2 Nerve growth factor 12 3.3 紅外燈與二養化碳培養箱 13 3.3.1 紅外燈機構 13 3.3.2 二養化碳培養箱 15 3.4 實驗細胞培養流程圖 17 3.5 實驗細胞處理及蛋白質濃度量測 19 3.6二維電泳 28 3.6.1原理 28 3.6.2二維電泳第一維─Isoelectric focusing(IEF) 29 3.6.3二維電泳第二維─Sodium dodecyl sulfate－polyacrylamide gel electrophoresis(SDS-PAGE) 33 3.7 Coomassie brilliant blue(CBR)染色和退色 38 3.8 蛋白質分析 40 3.9晶片製作 44 第4章 實驗結果 47 4.1細胞成長變化 47 4.2蛋白質變化分析 52 4.3晶片觀察與量測 60 第5章 結論與未來展望 61 參考文獻 63 | |
| dc.language.iso | zh-TW | |
| dc.title | 紅外線照射對PC12發育和基因表現影響及微電極晶片 | zh_TW |
| dc.title | Effect of Infrared Radiation on Rat PC12 Cells
Growth and Expression and MEA chip | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 郭柏齡(Po-Ling Kuo),潘建源(Chien-Yuan Pan) | |
| dc.subject.keyword | 紅外線,微電極晶片, | zh_TW |
| dc.subject.keyword | PC12,MEA chip, | en |
| dc.relation.page | 66 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2010-12-13 | |
| dc.contributor.author-college | 電機資訊學院 | zh_TW |
| dc.contributor.author-dept | 電子工程學研究所 | zh_TW |
| 顯示於系所單位： | 電子工程學研究所 | |
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