幾丁聚醣與聚麩胺酸摻混之水膠敷料於拔牙傷口之 活體評估

Abstract

幾丁聚醣與聚麩胺酸摻混之水膠敷料，是利用幾丁聚醣為一種無毒性且可在生物體內分解的聚合物，並有抗菌，促進傷口癒合以及幫助骨形成的作用;而聚麩胺酸則具有無毒性，良好生物相容性，吸水性佳等優點，將此水膠敷料置入拔牙傷口以達到幫助傷口癒合，骨生成之目的。本研究是應用大鼠之動物試驗模式，利用放射線及組織學觀察骨生成情形，水膠敷料於活體內降解速率及傷口癒合效果。將五十隻大鼠共一百顆上顎門齒隨機挑選六十四顆拔除，分成四組:不放任何敷料之控制組，放置臨床上常使用之商品止血敷料Spongostan之對照組，以及兩組試驗組:不含聚麩胺酸的幾丁聚醣敷料和幾丁聚醣與聚麩胺酸摻混之水膠敷料。之後分別在時間點一週，二週，四週，六週將大鼠犧牲作放射線及組織學觀察。結果顯示幾丁聚醣與聚麩胺酸摻混之水膠敷料相較於其他三組對組織沒有明顯不良反應，在骨生成方面六週時比控制組，對照組以及不含聚麩胺酸的幾丁聚醣效果為佳。

A novel chitosan/γPGA Hydrogel was composed of chitosan and γPGA because chitosan had antiseptic, biocompatible, and degradable properties and γPGA’s anionic nature allowed γPGA to form hydrogel with chitosan at an appropriate pH. The purpose of in-vivo study was to evaluate the chitosan/γPGA Hydrogel’s degradation rate, wound healing situation and new bone formation by radiography and histology. Fifty female Wistar rats (total 100 upper incisors) were used as the animal model. We chose randomly 64 extraction sockets and they were divided into 4 groups: no implant insertion(control group), Spongostan(control group), chitosan sponge(experiment group), chitosan/γPGA Hydrogel(experiment group).The rats were sacrificed later at the time interval of 1, 2 ,4, 6 weeks for further radiographic and histological evaluations. The results showed the chitosan/γPGA Hydrogel’s effect on new bone formation was better than other 3 groups.