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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林俊彬,謝國煌 | |
| dc.contributor.author | Yu-Horng Chuang | en |
| dc.contributor.author | 莊俞宏 | zh_TW |
| dc.date.accessioned | 2021-06-08T07:21:02Z | - |
| dc.date.copyright | 2008-08-13 | |
| dc.date.issued | 2008 | |
| dc.date.submitted | 2008-07-25 | |
| dc.identifier.citation | Aimin C, Chunlin H, Juliang B, Tinyin Z, Zhichao D (1999). Antibiotic loaded chitosan bar. An in vitro, in vivo study of a possible treatment for osteomyelitis. Clin Orthop Relat Res: 239-47.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/26690 | - |
| dc.description.abstract | 幾丁聚醣與聚麩胺酸摻混之水膠敷料，是利用幾丁聚醣為一種無毒性且可在生物體內分解的聚合物，並有抗菌，促進傷口癒合以及幫助骨形成的作用;而聚麩胺酸則具有無毒性，良好生物相容性，吸水性佳等優點，將此水膠敷料置入拔牙傷口以達到幫助傷口癒合，骨生成之目的。本研究是應用大鼠之動物試驗模式，利用放射線及組織學觀察骨生成情形，水膠敷料於活體內降解速率及傷口癒合效果。將五十隻大鼠共一百顆上顎門齒隨機挑選六十四顆拔除，分成四組:不放任何敷料之控制組，放置臨床上常使用之商品止血敷料Spongostan之對照組，以及兩組試驗組:不含聚麩胺酸的幾丁聚醣敷料和幾丁聚醣與聚麩胺酸摻混之水膠敷料。之後分別在時間點一週，二週，四週，六週將大鼠犧牲作放射線及組織學觀察。結果顯示幾丁聚醣與聚麩胺酸摻混之水膠敷料相較於其他三組對組織沒有明顯不良反應，在骨生成方面六週時比控制組，對照組以及不含聚麩胺酸的幾丁聚醣效果為佳。 | zh_TW |
| dc.description.abstract | A novel chitosan/γPGA Hydrogel was composed of chitosan and γPGA because chitosan had antiseptic, biocompatible, and degradable properties and γPGA’s anionic nature allowed γPGA to form hydrogel with chitosan at an appropriate pH. The purpose of in-vivo study was to evaluate the chitosan/γPGA Hydrogel’s degradation rate, wound healing situation and new bone formation by radiography and histology. Fifty female Wistar rats (total 100 upper incisors) were used as the animal model. We chose randomly 64 extraction sockets and they were divided into 4 groups: no implant insertion(control group), Spongostan(control group), chitosan sponge(experiment group), chitosan/γPGA Hydrogel(experiment group).The rats were sacrificed later at the time interval of 1, 2 ,4, 6 weeks for further radiographic and histological evaluations. The results showed the chitosan/γPGA Hydrogel’s effect on new bone formation was better than other 3 groups. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T07:21:02Z (GMT). No. of bitstreams: 1 ntu-97-R95450018-1.pdf: 2082868 bytes, checksum: b6bd80ffd24ff9c7fee3f6b2f36a0065 (MD5) Previous issue date: 2008 | en |
| dc.description.tableofcontents | 中文摘要 III
英文摘要 IV 目錄 V 表目錄 VIII 圖目錄 IX 第1章 前言 1 第2章 文獻回顧 2 2.1 拔牙後傷口癒合 2 2.2 骨移植替代材料 3 2.3引導組織再生以及引導骨再生 4 2.3.1 引導組織再生 4 2.3.2 引導骨再生 5 2.3.3 再生膜 6 2.4幾丁質和基丁聚醣 8 2.4.1 幾丁質 8 2.4.2 幾丁聚醣 9 2.5 聚麩胺酸(γPGA) 12 2.6 水膠 14 2.7 Spongostan 15 2.7.1 明膠 15 2.7.2 Spongostan 15 第3章 研究動機 17 第4章 材料與方法 19 4.1實驗材料及實驗儀器 19 4.1.1 Chitosan sponge 19 4.1.2 幾丁聚醣與聚麩胺酸摻混製成的水膠 20 4.1.3其他實驗器材 21 4.2實驗方法 22 4.2.1動物來源及飼養條件 22 4.2.2實驗設計及分組 22 4.2.2.1全身及局部麻醉 23 4.2.2.2拔除上顎門齒 23 4.2.2.3實驗分組 24 4.2.2.4拍攝X光片 24 4.2.2.5 X光片觀察與評估 25 4.2.2.6組織切片製作 25 4.2.2.7組織切片觀察 26 4.2.2.8組織切片評估 26 4.2.2.9統計與資料分析 27 第5章 結果 28 5.1放射線觀察 28 5.1.1 第一週 28 5.1.2 第二週 28 5.1.3 第四週 28 5.1.4 第六週 28 5.2 放射線評估 28 5.3 組織切片觀察 28 5.3.1 第一週 28 5.3.2 第二週 29 5.3.3 第四週 29 5.3.4 第六週 30 5.4 組織切片評估 31 5.5統計與資料分析 31 第6章 討論 32 第7章 結論與未來研究方向 37 參考文獻 38 表目錄 表5-1 放射線評估結果 44 表5-2 組織學評估結果 45 表5-3 六週時control與spongostan對於骨新生的關係 46 表5-4 六週時control與Chitosan sponge對於骨新生的關係 46 表5-5 六週時control與Chitosan/rPGA hydrogel對於骨新生的關係 47 表5-6 六週時spongostan與Chitosan sponge對於骨新生的關係 47 表5-7 六週時spongostan與Chitosan/rPGA hydrogel對於骨新生的關係 48 表5-8 六週時chitosan sponge與Chitosan/rPGA hydrogel對於骨新生的關係 48 圖目錄 圖5-1放射線觀察: 1週 49 圖5-2放射線觀察: 2週 50 圖5-3放射線觀察:4週 51 圖5-4放射線觀察:6週 52 圖5-5 控制組(拔牙窩內未放置任何敷料)一週後H&E染色情形 53 圖5-6 拔牙窩內放置spongostan一週後H&E染色情形 54 圖5-7 拔牙窩內放置chitosan sponge一週後H&E染色情形 55 圖5-8 拔牙窩內放置chitosan/γPGA hydrogel一週後H&E染色情形 56 圖5-9 控制組(拔牙窩內未放置任何敷料)二週後H&E染色情形 57 圖5-10拔牙窩內放置spongostan二週後H&E染色情形 58 圖5-11拔牙窩內放置chitosan sponge二週後H&E染色情形 59 圖5-12拔牙窩內放置chitosan/γPGA hydrogel二週後H&E染色情形 60 圖5-13 控制組(拔牙窩內未放置任何敷料)四週後H&E染色情形 61 圖5-14 拔牙窩內放置spongostan四週後H&E染色情形 62 圖5-15拔牙窩內放置chitosan sponge四週後H&E染色情形 63 圖5-16 拔牙窩內放置chitosan/γPGA hydrogel四週後H&E染色情形 64 圖5-17 控制組(拔牙窩內未放置任何敷料)六週後H&E染色情形 65 圖5-18 拔牙窩內放置spongostan六週後H&E染色情形 66 圖5-19拔牙窩內放置chitosan sponge 六週後H&E染色情形 67 圖5-20拔牙窩內放置chitosan/γPGA hydrogel六週後H&E染色情形 68 | |
| dc.language.iso | zh-TW | |
| dc.title | 幾丁聚醣與聚麩胺酸摻混之水膠敷料於拔牙傷口之
活體評估 | zh_TW |
| dc.title | In-vivo Evaluation of a Chitosan/γPGA Hydrogel
Dressing in Post-extraction Sockets | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 96-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林峰輝 | |
| dc.subject.keyword | 拔牙窩,幾丁聚醣,聚麩胺酸,水膠,Spongostan, | zh_TW |
| dc.subject.keyword | extraction sockets,chitosan,γPGA,hydrogel,Spongostan, | en |
| dc.relation.page | 68 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2008-07-25 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 口腔生物科學研究所 | zh_TW |
| 顯示於系所單位： | 口腔生物科學研究所 | |
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