天然矽片擔體奈米級氧化銅及氧化鋅粒子及其抗菌應用

Abstract

本研究利用片狀矽酸鹽黏土(NSP)來使奈米氧化鋅粒子(ZnO)、奈米氧化銅粒子(CuO)以及奈米銀粒子(Ag)穩定生成於矽片上，並合成奈米矽片氧化鋅(ZnO/NSP)、奈米矽片氧化銅(CuO/NSP)以及奈米矽片(Ag/NSP)，並利用紫外光−可見光光譜、X-ray粉末繞射儀以及穿透式電子顯微鏡(TEM)觀察奈米粒子的生成、粒子大小以及粒徑分佈。從實驗檢測結果發現，脫層的奈米矽片(NSP) 可承載以及穩定奈米粒子。奈米粒子/矽片之複合材料可成功地被合成出來，並可免除使用常見的有機分散劑所造成的過度包覆奈米粒子，使得奈米粒子的表面功能降低，而透過矽片可使奈米粒子表面露出，並達到提升奈米粒子功能的效果。奈米矽片擁有特殊的表面特性，例如:高比表面積以及高表面電荷，使得奈米矽片可以承載奈米粒子以及穩定分散奈米粒子。 我們進一步研究奈米複合物(奈米矽片氧化鋅(ZnO/NSP)、奈米矽片氧化銅(CuO/NSP))的殺菌效果，並和實驗室先前合成的奈米矽片銀(Ag/NSP)做比較。抗菌實驗採用大腸桿菌(E. coli)以及金黃色葡萄球菌(S. aureus) 作為試驗菌種。此外，我們合成奈米矽片銀/氧化鋅(Ag/ZnO/NSP)，其中奈米氧化鋅粒子視為奈米矽片銀的抗菌促進劑，並比較奈米矽片銀(Ag/NSP)以及奈米矽片銀/氧化鋅(Ag/ZnO/NSP)的抗菌性能。從抗菌結果發現到奈米矽片氧化銅(CuO/NSP)相較於奈米氧化鋅粒子(ZnO)以及奈米銀粒子(Ag)對金黃色葡萄球菌有較佳的抗菌效果。另外，含銀粒子的複合材料(Ag/NSP以及Ag/ZnO/NSP)則是對大腸桿菌有較佳的抗菌效果。另外，從菌數減低動態圖發現，奈米矽片銀/氧化鋅(Ag/ZnO/NSP)中的氧化鋅粒子可有效提升奈米矽片銀的抗菌效能。

The hybrids of zinc oxide, copper (II) oxide, silver oxide nanoparticles on the nanoscale silicate platelets (NSP) are synthesized and characterized by UV-visible spectroscopy, X-ray powder diffraction, and TEM. We employ the exfoliated silicate platelets to support and stabilize the nanoparticles. The particles-on-platelet nanohybrids are successfully synthesized and the naked nanoparticles associated with the NSPs are formed without organics contamination. It is suggested that the NSPs are suitable for supporting nanoparticles owing to their high aspect–ratio and high surface ionic charges. We further investigate the bactericidal efficacy of ZnO/NSP and CuO/NSP as compared with the home-made Ag/NSP at the weight ratio of 15/85. The antimicrobial tests are performed on Escherichia coli and Staphylococcus aureus. Additionally, we synthesize the Ag/ZnO/NSP that ZnO is regarded as a promoter in the hybrids in antibacterial uses. Also, the differences of antibacterial activities between Ag/NSP and Ag/ZnO/NSP are investigated. And, it is found that CuO/NSP (15/85, w/w) show better antibacterial efficacy against S. aureus as compared with ZnO/NSP and Ag/NSP. And the Ag-contained nanohybrids (Ag/NSP and Ag/ZnO/NSP) are excellent antibacterial agents for E. coli. Among the Ag/ZnO/NSP, ZnO promote the antibacterial activity of Ag/NSP.