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Title: | Ku80與EB病毒Zta蛋白質的功能研究 Ku80 and the Functions of Zta of Epstein-Barr Virus |
Authors: | Chien-Chang Chen 陳建彰 |
Advisor: | 張麗冠 |
Keyword: | 人類疱,疹病毒第四型,Zta,Ku80,溶裂期,DNA複製, Epstein-barr virus,Zta,Ku80,Lytic cycle,DNA replication, |
Publication Year : | 2009 |
Degree: | 碩士 |
Abstract: | EB病毒 (Epstein-Barr virus;EBV)屬於一種人類疱疹病毒,當病毒進入溶裂期會表現出極早期基因BZLF1並轉譯出Zta蛋白質,Zta為一個多功能蛋白質,主要負責調控基因轉錄及將DNA複製相關的蛋白質聚集到複製起始點。為了探討Zta在細胞中是否與其它細胞內蛋白質結合,對EB病毒溶裂期機制造成影響,所以本研究利用His pull-down assay吸附細胞蛋白質並以MALDI-TOF進行蛋白質定序發現人類的ATP-dependent DNA helicase II Ku80會與Zta結合,接著進一步以共同免疫沉澱分析和GST-pull down證實了Zta會與Ku80在細胞內及細胞外結合並一起結合到oriLyt上,並且觀察到Zta以C端區域與Ku80的N端區域結合,也在Ku80缺陷的P3HR1細胞中觀察到EB病毒溶裂期DNA複製的下降,Ku80也會與Zta和EA-D在細胞內結合。此外,利用冷光分析發現Ku80會增加Zta的轉錄活性。綜合以上,Zta與Ku80結合後會進一步調控EB病毒溶裂期複製。 Epstein-Barr virus (EBV) expresses immediate-early transcription factor Zta during the lytic cycle. Zta is a multifunctional protein that regulates gene transcription and recruits a replication complex at the lytic viral origin of replication. To identify cellular proteins that associate with Zta, this study performed His-Zta pull down analysis to precipitate cellular proteins and identified by MALDI-TOF a human ATP-dependent DNA helicase II Ku80 that interacts with Zta. Furthermore, Zta interacts with Ku80 in vivo and in vitro, forming a complex that binds to ZREs at the oriLyt. Furthermore, depleting Ku80 reduces EBV lytic DNA replication, showing the involvement of Ku80 in EBV lytic development. This study shows that the C-terminal domain of Zta interacts with the N- and M-domain of Ku80. Ku80 and Zta also interact with BMRF1 polymerase processivity factor, suggesting that Ku80 facilitates viral lytic DNA replication . In addition, Ku80 enhances the transcriptional activity of Zta. Taken together, this study found Ku80 involves in EBV lytic replication through an interaction with Zta. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/9018 |
Fulltext Rights: | 同意授權(全球公開) |
Appears in Collections: | 微生物學科所 |
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ntu-98-1.pdf | 1 MB | Adobe PDF | View/Open |
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