以液相層析串聯質譜法分析糖漿產品中活性雙羰基化合物

Abstract

活性雙羰基物質(reactive dicarbonyl species, RDS)為醣類降解物，容易在焦糖化反應及梅納反應中生成，被認為是造成代謝相關疾病的原因之一。市售糖漿在製作過程中使用的熱處理可能產生大量的RDS。為了瞭解市售糖漿中RDS的含量及分布，本研究利用o-phenylenediamine將RDS衍生成quinoxaline類化合物，搭配液相層析串聯質譜法同步分析市售糖漿中16種RDS，其中包含glyoxal及methylglyoxal。除此之外亦針對分析方法進行最適化，包含空白樣品、衍生劑品牌、衍生條件、淨化試劑、RDS衍生物穩定度及分析管柱的選擇。RDS總含量在高果糖糖漿(754.5-1318 μg/g)及龍舌蘭糖漿(大於1024 μg/g)中較高；葡萄糖糖漿(0.625-78.99 μg/g)、麥芽糖糖漿(8.413-10.30 μg/g)及楓糖漿(2.511-17.81 μg/g)則較低；葡萄糖、果糖及蔗糖中亦可偵測到RDS (trace-177.6 μg/g)。本分析方法未來可用於分析市售糖漿在製作及儲藏過程中RDS的分布及變化，並對市售產品進行品質控管。

Reactive dicarbonyl species (RDS), the degradation products of saccharides in caramelization and Maillard reactions, are considered to be related to the diabetic complications and chronic diseases. It can be expected that abundant RDS emerge in the commercial syrups due to the thermal processing. In order to monitor RDS in commercial syrups, RDS are derivatized with o-phenylenediamine to quinoxaline derivatives. There are 16 RDS analyzed simultaneously by LC-MS/MS, including glyoxal and methylglyoxal. In addition, this analytical method is optimized, including blank, the brand of derivatization reagent, derivatization conditions, clean-up solvents, stability of quinoxaline derivative, and analytical column. Contents of total RDS are higher in high-fructose syrups (754.5-1318 μg/g) and agave syrups (beyond 1024 μg/g) than that in glucose syrups (0.625-78.99 μg/g), maltose syrups (8.413-10.30 μg/g), and maple syrups (2.511-17.81 μg/g). RDS are also detected in glucose, fructose and sucrose (trace-177.6 μg/g). This analytical method can be applied to understand the distribution and change of contents of RDS in the commercia syrups during the production process and storage, and further monitor the quality of syrups.