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標題: | 阿拉伯芥中FIN219與Cryptochrome 2在藍光及茉莉酸訊息傳遞途徑中之功能性探討 Functional Studies of FIN219 and Cryptochrome 2 in the Integration of Blue Light and Jasmonate Signaling in Arabidopsis |
作者: | Wen-Zhe Gu 顧聞喆 |
指導教授: | 謝旭亮 |
關鍵字: | FIN219,隱花色素2,茉莉酸,藍光, FIN219,Cryptochrome 2,Jasmonic Acid,Blue Light, |
出版年 : | 2017 |
學位: | 碩士 |
摘要: | 光和植物荷爾蒙在植物的生長發育調控過程中皆扮演重要角色,植物整合這兩方面的訊息傳遞路徑之機制仍有待探究。阿拉伯芥中的隱花色素2(Cryptochrome 2(CRY2))為其藍光受體之一,參與藍光下光形態發生和開花時間的調控,藍光活化的CRY2將受到CONSTITUTIVE PHOTOMORPHOGENIC1(COP1)的泛素化標記和分解。Far-red insensitive 219 (FIN219)是參與茉莉酸訊息傳遞中的酵素,能將茉莉酸(Jasmonic Acid(JA))合成為具有生物活性的Jasmonoyl- isoleucine (JA-Ile)。它亦在光敏素A介導的遠紅光訊息傳遞路徑中作為一個正調控因子。前人研究顯示FIN219同時參與在CRY2相關的藍光訊息傳遞路徑中。大量表現FIN219蛋白質的阿拉伯芥幼苗在藍光下呈現較短的下胚軸,而在CRY2缺失突變株的背景中大量表現FIN219蛋白質時,植株幼苗並不會表現對藍光敏感,且FIN219蛋白質的大量表達亦會促進阿拉伯芥提早開花。為了進一步了解FIN219與CRY2的交互作用對茉莉酸及藍光訊息傳遞的影響,本研究重新確認了FIN219與CRY2在藍光下存在直接的交互作用,且發現了FIN219蛋白質的大量表現可以增加CRY2蛋白質在藍光下的穩定性,而外加甲基茉莉酸(MeJA)則會使CRY2蛋白質在藍光下更不穩定。另外,發現了FIN219與CRY2共同調控藍光下阿拉伯芥下胚軸的光形態形成,在有FIN219蛋白質存在時,CRY2蛋白質的存在能抑制外加甲基茉莉酸對下胚軸伸長的抑制。在對於開花時間的調控方面,短日照條件下,大量表現FIN219基因的阿拉伯芥植株中可以偵測到更多的Flowering Locus T (FT)基因。然而,我們仍需要更多的證據來證實FIN219在其中所扮演的角色。綜合上述, FIN219參與在CRY2所調控的藍光訊息傳遞路徑中,並促進阿拉伯芥之光形態發生。 Light and plant hormones play important roles in the regulation of plant growth and development. Cryptochrome 2 (CRY2) is one of the blue light (BL) receptors that mediate de-etiolation primarily under weak blue light and photoperiodic control of flowering in Arabidopsis. Under BL stimulus, CRY2 is quickly ubiquitinated and degraded by CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1). FAR-RED INSENSITIVE219 (FIN219) catalyzes the biosynthesis of Jasmonoyl-isoleucine (JA-Ile), a bio-active form of Jasmonic Acid (JA), and is a positive regulator of phytochrome A (phyA) mediated far-red light signaling. Previous studies showed that FIN219 was also involved in CRY2-mediated blue light signaling. The overexpression of FIN219 would resulted in a hypersensitive phenotype in response to blue light, ectopic expression of FIN219 in cry2 mutant did not exhibit obvious phenotype, and overexpression of FIN219 would lead to early flowering phenotype. This study verified the physical interaction between CRY2 and FIN219 protein under blue light, and observed that the CRY2 protein stability decreased with MeJA treatment but increased with the overexpression of FIN219 under blue light. CRY2 and FIN219 functioned together in BL-mediated inhibition of hypocotyl elongation, and CRY2 alleviated the inhibition of hypocotyl elongation caused by MeJA depending on FIN219. In short day (SD) condition, more Flowering Locus T (FT) gene was detected in FIN219 overexpression line. However, more evidence was needed to elucidate the role of FIN219 in CRY2-mediate regulation of flowering. Taken together, FIN219 is involved in CRY2-mediated blue light signaling pathway, and promotes photomorphogenesis in Arabidopsis. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/7758 |
DOI: | 10.6342/NTU201701965 |
全文授權: | 同意授權(全球公開) |
顯示於系所單位: | 植物科學研究所 |
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