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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 謝旭亮 | |
dc.contributor.author | Wen-Zhe Gu | en |
dc.contributor.author | 顧聞喆 | zh_TW |
dc.date.accessioned | 2021-05-19T17:52:34Z | - |
dc.date.available | 2022-07-27 | |
dc.date.available | 2021-05-19T17:52:34Z | - |
dc.date.copyright | 2017-07-27 | |
dc.date.issued | 2017 | |
dc.date.submitted | 2017-07-25 | |
dc.identifier.citation | Ahmad, M., Jarillo, J., A., Cashmore, A., R. (1998a). Chimeric proteins between cry1 and cry2 Arabidopsis blue light photoreceptors indicate overlapping functions and varying protein stability. Plant Cell 10: 197–208.
Ahmad, M., Jarillo, J., A., Smirnova, O., Cashmore, A., R. (1998b). The CRY1 blue light photoreceptor of Arabidopsis interacts with phytochrome A in vitro. Mol Cell 1: 939–948. Andrés, F., and Coupland, G. (2012). The genetic basis of flowering responses to seasonal cues. Nature Reviews Genetics 13: 627–639. Arsovski, A. A., Galstyan, A., Guseman, J. M., and Nemhauser, J., L. (2012). Photomorphogenesis. The Arabidopsis Book e0147. Banerjee, R., Schleicher, E., Meier, S., Viana, R., M., Pokorny, R., Ahmad, M., Bittl, R., Batschauer, A. (2007). The signaling state of Arabidopsis cryptochrome 2 contains flavin semiquinone. J Biol Chem 282:14916–14922. Bolouri Moghaddam, M., R., and Van den Ende, W. (2013). Sugars, the clock and transition to flowering. Front Plant Sci 4: 22. Briggs, W. R., and Christie, J. M. (2002). Phototropins 1 and 2: versatile plant blue-light receptors. Trends in Plant science 7: 204-210. Campos-Rivero, G., Osorio-Montalvo, P., Sánchez-Borges, R., Us-Camas, R., Duarte-Aké, F., and De-la-Peña, C. (2017). Plant hormone signaling in flowering: an epigenetic point of view. Journal of Plant Physiology 214: 16. Carvalhais, L. C., Schenk, P. M., and Dennis, P. G. (2017). Jasmonic acid signalling and the plant holobiont. Current Opinion in Microbiology 3: 42-47. Chang, Tzu-Huang. (2015). Functional Studies of Arabidopsis FAR-RED INSENSITIVE219 and Cryptochrome2 interaction in the integration of blue light and JA signaling. National Taiwan University Master Thesis. Chen, C., Huang, I. C., Liu, M. J., Wang, Z. G., Chung, S. S., and Hsieh, H. L. (2007). Glutathione S-transferase interacting with far-red insensitive 219 is involved in phytochrome A-mediated signaling in Arabidopsis. Plant Physiology 143: 1189-1202. Chen, C. Y., Ho, S. S., Kuo, T. Y., Hsieh, H. L., and Cheng, Y. S. (2017). Structural basis of jasmonate-amido synthetase FIN219 in complex with glutathione S-transferase FIP1 during the JA signal regulation. Proceedings of the National Academy of Sciences 114: 1815-1824. Deng, X. W., and Quail, P., H. (1999). Signalling in light-controlled development. In Seminars in Cell & Developmental Biology 10: 121-129. Du, Xin-Jie. (2005). Investigation of the Involvement of Arabidopsis FIN219 in the Integration of Blue Light and Ethylene Signaling Pathways. National Taiwan University Master Thesis. Duek, P., D., Elmer, M., V., van Oosten, V.R., and Fankhauser, C. (2004). The degradation of HFR1, a putative bHLH class transcription factor involved in light signaling, is regulated by phosphorylation and requires COP1. Current Biology 14: 2296-2301. Fairchild, C. D., Schumaker, M.A., and Quail, P.H. (2000). HFR1 encodes an atypical bHLH protein that acts in phytochrome A signal transduction. Genes Dev 14: 2377-2391. Fornara, F., de Montaigu, A., and Coupland, G. (2010). SnapShot: Control of flowering in Arabidopsis. Cell 141: 550–550. Fornara, F., Panigrahi, K. C., Gissot, L., Sauerbrunn, N., Rühl, M., Jarillo, J.A. and Coupland, G. (2009). Arabidopsis DOF transcription factors act redundantly to reduce CONSTANS expression and are essential for a photoperiodic flowering response. Development Cell 17: 75–86. Fu, Tsu-Yu. (2008). Functional studies of Arabidopsis FIN219 in cross-talks between blue light and far-red light signaling. National Taiwan University Master Thesis. Helliwell, C.A., Wood, C.C., Robertson, M., James Peacock, W., and Dennis, E.S. (2006). The Arabidopsis FLC protein interacts directly in vivo with SOC1 and FT chromatin and is part of a highmolecular- weight protein complex. Plant Journal 46: 183–192. He, S. B., Wang, W. X., Zhang, J. Y., Xu, F., Lian, H. L., Li, L., Yang, H. Q. (2015). The CNT1 domain of Arabidopsis CRY1 alone is sufficient to mediate blue light inhibition of hypocotyl elongation. Molecular Plant 8:822–825. Hoang, N., Bouly, J. P., Ahmad, M. (2008). Evidence of a light-sensing role for folate in Arabidopsis cryptochrome blue-light receptors. Mol Plant 1:68–74 Hornitschek, P., Lorrain, S., Zoete, V., Michielin, O., and Fankhauser, C. (2009). Inhibition of the shade avoidance response by formation of non-DNA binding bHLH heterodimers. The EMBO Journal 28: 3893-3902. Hsieh, H. L., Okamoto, H., Wang, M., Ang, L. H., Matsui, M., Goodman, H., and Deng, X. W. (2000). FIN219, an auxin-regulated gene, defines a link between phytochrome A and the downstream regulator COP1 in light control of Arabidopsis development. Genes & Development 14: 1958-1970. Jang, I.C., Henriques, R., Seo, H.S., Nagatani, A. and Chua, N.H. (2010). Arabidopsis PHYTOCHROME INTERACTING FACTOR proteins promote phytochrome B polyubiquitination by COP1 E3 ligase in the nucleus. Plant Cell 22: 2370-2383. Kardailsky, I., Shukla, V.K., Ahn, J.H., Dagenais, N., Christensen, S.K., Nguyen, J.T., Chory, J., Harrison, M.J. and Weigel, D. (1999). Activation tagging of the floral inducer FT. Science 286: 1962–1965. Kennedy, M. J., Hughes, R. M., Peteya, L. A., Schwartz, J. W., Ehlers, M. D. and Tucker, C. L. (2010). Rapid blue-light-mediated induction of protein interactions in living cells. Nature Methods 7: 973–975. Kronenberg, G. H. and Kendrick, R. E. (1994). Photomorphogenesis in plants. Leivar, P. and Quail, P.H. (2011). PIFs: pivotal components in a cellular signaling hub. Trends in Plant Science 16: 19-28. Lian, H. L., He, S. B., Zhang, Y.C., Zhu, D.M., Zhang, J.Y., Jia, K. P., Sun, S. X., Li, L., Yang, H. Q. (2011). Blue-light-dependent interaction of cryptochrome 1 with SPA1 defines a dynamic signaling mechanism. Genes & Development 25:1023–1028. Liu, B., Zuo, Z., Liu, H., Liu, X., Lin, C. (2011). Arabidopsis cryptochrome 1 interacts with SPA1 to suppress COP1 activity in response to blue light. Genes & Development 25:1029–1034. Liu, H., Liu, B., Zhao, C., Pepper, M., Lin, C. (2011). The action mechanisms of plant cryptochromes. Trends in Plant Science 16: 684-691. Liu, H., Yu, X., Li, K., Klejnot, J., Yang, H., Lisiero, D., Lin, C. (2008). Photoexcited CRY2 interacts with CIB1 to regulate transcription and floral initiation in Arabidopsis. Science 322: 1535-1539. Liu, Y., Li, X., Li, K., Liu, H., Lin, C. (2013). Multiple bHLH proteins form heterodimers to mediate CRY2-dependent regulation of flowering- time in Arabidopsis. PLoS Genetics 9: e1003861. Liu, Q., Wang, Q., Liu, B., Wang, W., Wang, X., Park, J., and Lin, C. (2016a). The blue light-dependent polyubiquitination and degradation of Arabidopsis Cryptochrome2 requires multiple E3 ubiquitin ligases. Plant and Cell Physiology 57: 2175-2186. Liu, B., Yang, Z., Gomez, A., Liu, B., Lin, C., and Oka, Y. (2016b). Signaling mechanisms of plant cryptochromes in Arabidopsis thaliana. Journal of Plant Research 129: 137. Lo, Yi-Ying. (2015). Functional Studies of FIN219, COP1 and COI1 Proteins in the Integration of Far-red Light and Jasmonate Signaling in Arabidopsis. National Taiwan University Master Thesis. Malhotra, K., Kim, S.T., Batschauer, A., Dawut, L., and Sancar, A. (1995). Putative blue-light photoreceptors from Arabidopsis thaliana and Sinapis alba with a high degree of sequence homology to DNA photolyase contain the two photolyase cofactors but lack DNA repair activity. Biochemistry 34: 6892–6899. Mathieu, J., Yant, L.J., Mürdter, F., Küttner, F., and Schmid, M. (2009). Repression of flowering by the miR172 target SMZ. PLoS Biology 7: e1000148. Más, P., Devlin, P. F., Panda, S., and Kay, S. A. (2000). Functional interaction of phytochrome B and cryptochrome 2. Nature 408: 207–211 Mockler, T.C., Guo, H., Yang, H., Duong, H., and Lin, C. (1999). Antagonistic actions of Arabidopsis cryptochromes and phytochrome B in the regulation of floral induction. Development 126(10): 2073–2082 Mockler, T., Yang, H., Yu, X., Parikh, D., Cheng, Y.C., Dolan, S., and Lin, C. (2003) Regulation of photoperiodic flowering by Arabidopsis photoreceptors. Proc Natl Acad Sci USA 100: 2140–2145 Osterlund, M.T., Hardtke, C.S., Wei, N., and Deng, X.W. (2000). Targeted destabilization of HY5 during light-regulated development of Arabidopsis. Nature 405: 462-466. Oyama, T., Shimura, Y., and Okada, K. (1997). The Arabidopsis HY5 gene encodes a bZIP protein that regulates stimulus-induced development of root and hypocotyl. Genes Development 11: 2983-2995. Pérez, A. C., and Goossens, A. (2013). Jasmonate signalling: a copycat of auxin signalling?. Plant, cell & environment 36(12): 2071-2084. Pokorny, R., Klar, T., Hennecke, U., Carell, T., Batschauer, A., and Essen, L.O. (2008). Recognition and repair of UV lesions in loop structures of duplex DNA by DASH-type cryptochrome. Proc Natl Acad Sci USA 105: 21023–21027. Putterill J, Robson F, Lee K, Simon R, Coupland G (1995) The CONSTANS gene of Arabidopsis promotes flowering and encodes a protein showing similarities to zinc finger transcription factors. Cell 80: 847–857. Riboni, M., Robustelli Test, A., Galbiati, M., Tonelli, C., and Conti, L. (2014). Environmental stress and flowering time: The photoperiodic connection. Plant Signaling & Behavior 9: e29036. Ritz, T., Yoshii, T., Helfrich-Foerster, C., and Ahmad, M. (2010). Cryptochrome: a photoreceptor with the properties of a magnetoreceptor? Commun Integr Biol 3: 24–27. Rizzini, L., Favory, J. J., Cloix, C., Faggionato, D., O’Hara, A., Kaiserli, E., and Ulm, R. (2011). Perception of UV-B by the Arabidopsis UVR8 protein. Science 332: 103-106. Rosenfeldt, G., Viana, R. M., Mootz, H. D., von Arnim, A. G., and Batschauer, A. (2008). Chemically induced and light-independent cryptochrome photoreceptor activation. Mol Plant 1: 4–14. Sang, Y., Li, Q. H., Rubio, V., Zhang, Y. C., Mao, J., Deng, X. W., and Yang, H. Q. (2005). N-terminal domain-mediated homodimerization is required for photoreceptor activity of Arabidopsis CRYPTOCHROME 1. Plant Cell 17:1569–1584. Schaller, A. and Stintzi, A. (2009). Enzymes in jasmonate biosynthesis–structure, function, regulation. Phytochemistry 70: 1532-1538. Shalitin, D., Yang, H., Mockler, T. C., Maymon, M., Guo, H., Whitelam, G. C., and Lin C. (2002). Regulation of Arabidopsis cryptochrome 2 by blue-light-dependent phosphorylation. Nature 417: 763–767. Shalitin, D., Yu, X., Maymon, M., Mockler, T., Lin, C. (2003). Blue lightdependent in vivo and in vitro phosphorylation of Arabidopsis cryptochrome 1. Plant Cell 15:2421–2429. Shin, J., Kim, K., Kang, H., Zulfugarov, I.S., Bae, G., Lee, C.H., Lee, D., and Choi, G. (2009). Phytochromes promote seedling light responses by inhibiting four negatively-acting phytochrome-interacting factors. Proc Natl Acad Sci USA 106: 7660-7665. Smyth, D.R., Bowman, J.L., Meyerowitz, E.M. (1990). Early flower development in Arabidopsis. Plant Cell 2: 755–767. Song, Y.H., Ito, S., and Imaizumi, T. (2013). Flowering time regulation: photoperiod- and temperature-sensing in leaves. Trends in Plant Science 18:575–583. Srikanth, A., and Schmid, M. (2011). Regulation of flowering time: all roads lead to Rome. Cellular and molecular life sciences 68: 2013-2037. Staswick, P. E., Tiryaki, I., and Rowe, M. L. (2002). Jasmonate response locus JAR1 and several related Arabidopsis genes encode enzymes of the firefly luciferase superfamily that show activity on jasmonic, salicylic, and indole-3-acetic acids in an assay for adenylation. Plant Cell 14: 1405-1415. Sullivan, J. A. and Deng, X. W. (2003). From seed to seed: the role of photoreceptors in Arabidopsis development. Developmental biology 260: 289-297. Tan, S. T., Dai, C., Liu, H. T., and Xue, H. W. (2013). Arabidopsis casein kinase1 proteins CK1.3 and CK1.4 phosphorylate cryptochrome2 to regulate blue light signaling. Plant Cell 25: 2618–2632. Usami, T., Mochizuki, N., Kondo, M., Nishimura, M., and Nagatani, A. (2004). Cryptochromes and phytochromes synergistically regulate Arabidopsis root greening under blue light. Plant Cell Physiology 45:1798–1808. Wasternack, C. and Song, S. (2016). Jasmonates: biosynthesis, metabolism, and signaling by proteins activating and repressing transcription. Journal of Experimental Botany 68: 1303-1321. Wang, J. G., Chen, C. H., Chien, C., T., and Hsieh, H. L. (2011). FAR-RED INSENSITIVE219 modulates CONSTITUTIVE PHOTOMORPHOGENIC1 activity via physical interaction to regulate hypocotyl elongation in Arabidopsis. Plant Physiology 156: 631-646. Wang, H., Ma, L., G., Li, J., M., Zhao, H., Y., and Deng, X., W. (2001). Direct interaction of Arabidopsis cryptochromes with COP1 in light control development. Science 294:154–158. Yang, H., Q., Tang, R., H., and Cashmore, A., R. (2001). The signaling mechanism of Arabidopsis CRY1 involves direct interaction with COP1. Plant Cell 13:2573–2587. Yang, H., Q., Wu, Y., J., Tang, R., H., Liu, D., Liu, Y., and Cashmore, A., R. (2000). The C termini of Arabidopsis cryptochromes mediate a constitutive light response. Cell 103:815-827. Yi, C. and Deng, X., W. (2005). COP1-from plant photomorphogenesis to mammalian tumorigenesis. Trends Cell Biol 15: 618–625. Zhai, Q., Zhang, X., Wu, F., Feng, H., Deng, L., Xu, L. and Li, C. (2015). Transcriptional mechanism of jasmonate receptor COI1-mediated delay of flowering time in Arabidopsis. Plant Cell 27: 2814-2828. Zuo, Z., Liu, H., Liu, B., Liu, X., and Lin, C. (2011). Blue light-dependent interaction of CRY2 with SPA1 regulates COP1 activity and floral initiation in Arabidopsis. Current Biology 21:841–847. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/7758 | - |
dc.description.abstract | 光和植物荷爾蒙在植物的生長發育調控過程中皆扮演重要角色,植物整合這兩方面的訊息傳遞路徑之機制仍有待探究。阿拉伯芥中的隱花色素2(Cryptochrome 2(CRY2))為其藍光受體之一,參與藍光下光形態發生和開花時間的調控,藍光活化的CRY2將受到CONSTITUTIVE PHOTOMORPHOGENIC1(COP1)的泛素化標記和分解。Far-red insensitive 219 (FIN219)是參與茉莉酸訊息傳遞中的酵素,能將茉莉酸(Jasmonic Acid(JA))合成為具有生物活性的Jasmonoyl- isoleucine (JA-Ile)。它亦在光敏素A介導的遠紅光訊息傳遞路徑中作為一個正調控因子。前人研究顯示FIN219同時參與在CRY2相關的藍光訊息傳遞路徑中。大量表現FIN219蛋白質的阿拉伯芥幼苗在藍光下呈現較短的下胚軸,而在CRY2缺失突變株的背景中大量表現FIN219蛋白質時,植株幼苗並不會表現對藍光敏感,且FIN219蛋白質的大量表達亦會促進阿拉伯芥提早開花。為了進一步了解FIN219與CRY2的交互作用對茉莉酸及藍光訊息傳遞的影響,本研究重新確認了FIN219與CRY2在藍光下存在直接的交互作用,且發現了FIN219蛋白質的大量表現可以增加CRY2蛋白質在藍光下的穩定性,而外加甲基茉莉酸(MeJA)則會使CRY2蛋白質在藍光下更不穩定。另外,發現了FIN219與CRY2共同調控藍光下阿拉伯芥下胚軸的光形態形成,在有FIN219蛋白質存在時,CRY2蛋白質的存在能抑制外加甲基茉莉酸對下胚軸伸長的抑制。在對於開花時間的調控方面,短日照條件下,大量表現FIN219基因的阿拉伯芥植株中可以偵測到更多的Flowering Locus T (FT)基因。然而,我們仍需要更多的證據來證實FIN219在其中所扮演的角色。綜合上述, FIN219參與在CRY2所調控的藍光訊息傳遞路徑中,並促進阿拉伯芥之光形態發生。 | zh_TW |
dc.description.abstract | Light and plant hormones play important roles in the regulation of plant growth and development. Cryptochrome 2 (CRY2) is one of the blue light (BL) receptors that mediate de-etiolation primarily under weak blue light and photoperiodic control of flowering in Arabidopsis. Under BL stimulus, CRY2 is quickly ubiquitinated and degraded by CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1). FAR-RED INSENSITIVE219 (FIN219) catalyzes the biosynthesis of Jasmonoyl-isoleucine (JA-Ile), a bio-active form of Jasmonic Acid (JA), and is a positive regulator of phytochrome A (phyA) mediated far-red light signaling. Previous studies showed that FIN219 was also involved in CRY2-mediated blue light signaling. The overexpression of FIN219 would resulted in a hypersensitive phenotype in response to blue light, ectopic expression of FIN219 in cry2 mutant did not exhibit obvious phenotype, and overexpression of FIN219 would lead to early flowering phenotype. This study verified the physical interaction between CRY2 and FIN219 protein under blue light, and observed that the CRY2 protein stability decreased with MeJA treatment but increased with the overexpression of FIN219 under blue light. CRY2 and FIN219 functioned together in BL-mediated inhibition of hypocotyl elongation, and CRY2 alleviated the inhibition of hypocotyl elongation caused by MeJA depending on FIN219. In short day (SD) condition, more Flowering Locus T (FT) gene was detected in FIN219 overexpression line. However, more evidence was needed to elucidate the role of FIN219 in CRY2-mediate regulation of flowering. Taken together, FIN219 is involved in CRY2-mediated blue light signaling pathway, and promotes photomorphogenesis in Arabidopsis. | en |
dc.description.provenance | Made available in DSpace on 2021-05-19T17:52:34Z (GMT). No. of bitstreams: 1 ntu-106-R03b42031-1.pdf: 3176022 bytes, checksum: be708fffcb1c062e50285c2da504e9cd (MD5) Previous issue date: 2017 | en |
dc.description.tableofcontents | INTRODUCTION 1
1. Light and Plants 1 2. Photomorphogenesis 1 3. Flowering 3 4. Cryptochromes and BL signaling 4 5. FIN219 and JA signaling 9 6. Crosstalks between light and JA signaling 11 MATERIALS AND METHODS 13 Plant materials and Growth conditions 13 Protein isolation and Western Blotting 14 Recombinant protein purification and Pull-down assays 15 Co-immunoprecipitation Assay 16 Protoplast transfection and BiFC Analysis 16 Hypocotyl length measurements 18 Flowering-time measurement 18 RNA isolation and Quantitative real-time PCR 18 RESULTS 20 1 Arabidopsis FIN219 physically interacts with the C-terminal of CRY2. 20 2 CRY2 and FIN219 act synergistically in BL-mediated inhibition of hypocotyl elongation. 21 3 CRY2 inhibits JA sensitivity depending on FIN219 22 4 FIN219 and CRY2 protein level under blue light conditions and MeJA treatment 23 4.1 FIN219 level was not directly influenced by CRY2 23 4.2 FIN219 increased CRY2 protein accumulation under blue light. 24 4.3 CRY2 protein stability was decreased by MeJA treatment under blue light. 25 5 Effect of FIN219 and CRY2 on flowering time under LD and SD conditions. 26 DISCUSSION 29 1. FIN219 physically interacts with BL-activated CRY2. 29 2. The regulation between FIN219 and CRY2 proteins in photomorphogenesis. 29 3. The crosstalk between CRY2 and JA response in photomorphogenesis. 31 4. Possible roles of FIN219 in flowering regulation. 33 CONCLUSION 34 FIGURES 36 REFERENCES 44 | |
dc.language.iso | en | |
dc.title | 阿拉伯芥中FIN219與Cryptochrome 2在藍光及茉莉酸訊息傳遞途徑中之功能性探討 | zh_TW |
dc.title | Functional Studies of FIN219 and Cryptochrome 2 in the Integration of Blue Light and Jasmonate Signaling in Arabidopsis | en |
dc.type | Thesis | |
dc.date.schoolyear | 105-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 鄭秋萍,鄭貽生,張英?,王雅筠 | |
dc.subject.keyword | FIN219,隱花色素2,茉莉酸,藍光, | zh_TW |
dc.subject.keyword | FIN219,Cryptochrome 2,Jasmonic Acid,Blue Light, | en |
dc.relation.page | 54 | |
dc.identifier.doi | 10.6342/NTU201701965 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2017-07-26 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
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