明膠?在雌鼠子宮中動情週期的表現

Abstract

成熟雌鼠排卵、受精卵著床、分娩及子宮組織在動情週期的重組等生理變化過程中，都需要胞外間質成份的降解和重新合成。胞外間質帶金屬離子蛋白?(MMP)家族成員則是擔任調控胞外間質平衡的重要角色。胞外間質帶金屬離子蛋白?以潛伏蛋白(proenzyme)的形式分泌到細胞外進行活化，其活性會受到專一性的胞外金屬?抑制蛋白(TIMP）所調控。 本論文中，我們觀察MMP家族中的gelatinase B(MMP-9)基因和蛋白在雌鼠子宮動情週期各階段表現。從 Zymography 和RT-PCR的結果顯示，gelatinase B在動情前期和動情期有較強的表現，而動情間期的表現減少，動情後期則幾乎不表現。Gelatinase B的表現可能受到黃體素的抑制。我們推論gelatinase B在雌鼠子宮動情前期和動情期的表現可能會參與子宮組織降解和重組，而TIMP-1在動情週期的表現幾乎沒有變化。

In the adult female, some of the most actively changing tissues are the reproductive organs. For example, ovulation, implantation, parturition, and uterus continually remodels throughout the estrous cycle. All of these processes require both breakdown and resynthesis of the extracellular matrix (ECM) components. Matrix metalloproteinase (MMP) are believed to be primary contributors to the process. Secreted as proenzymes, the MMPs undergo conversion to a catalytic active form that can be regulated by specific endogenous tissue inhibitors of metalloproteinases (TIMPs). The results of Zymography and RT-PCR revealed that the expression level of gelatinase B appeared intensively at proestrus and estrus during mouse estrous cycle, declined sharply at metestrus, and nearly undetected in diestrus. The expression of gelatinase B may be inhibited by progesterone. We supposed that the expression of gelatinase B in proestrus and estrus might be involved in the tissue remodeling processes of uterus. The expression of TIMP-1 in uterus during mouse estrous cycle was constant.