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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Jun-An Chen | en |
| dc.contributor.author | 陳俊安 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:11:22Z | - |
| dc.date.available | 2021-07-01T08:11:22Z | - |
| dc.date.issued | 1999 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75015 | - |
| dc.description.abstract | 成熟雌鼠排卵、受精卵著床、分娩及子宮組織在動情週期的重組等生理變化過程中，都需要胞外間質成份的降解和重新合成。胞外間質帶金屬離子蛋白?(MMP)家族成員則是擔任調控胞外間質平衡的重要角色。胞外間質帶金屬離子蛋白?以潛伏蛋白(proenzyme)的形式分泌到細胞外進行活化，其活性會受到專一性的胞外金屬?抑制蛋白(TIMP）所調控。 本論文中，我們觀察MMP家族中的gelatinase B(MMP-9)基因和蛋白在雌鼠子宮動情週期各階段表現。從 Zymography 和RT-PCR的結果顯示，gelatinase B在動情前期和動情期有較強的表現，而動情間期的表現減少，動情後期則幾乎不表現。Gelatinase B的表現可能受到黃體素的抑制。我們推論gelatinase B在雌鼠子宮動情前期和動情期的表現可能會參與子宮組織降解和重組，而TIMP-1在動情週期的表現幾乎沒有變化。 | zh_TW |
| dc.description.abstract | In the adult female, some of the most actively changing tissues are the reproductive organs. For example, ovulation, implantation, parturition, and uterus continually remodels throughout the estrous cycle. All of these processes require both breakdown and resynthesis of the extracellular matrix (ECM) components. Matrix metalloproteinase (MMP) are believed to be primary contributors to the process. Secreted as proenzymes, the MMPs undergo conversion to a catalytic active form that can be regulated by specific endogenous tissue inhibitors of metalloproteinases (TIMPs). The results of Zymography and RT-PCR revealed that the expression level of gelatinase B appeared intensively at proestrus and estrus during mouse estrous cycle, declined sharply at metestrus, and nearly undetected in diestrus. The expression of gelatinase B may be inhibited by progesterone. We supposed that the expression of gelatinase B in proestrus and estrus might be involved in the tissue remodeling processes of uterus. The expression of TIMP-1 in uterus during mouse estrous cycle was constant. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:11:22Z (GMT). No. of bitstreams: 0 Previous issue date: 1999 | en |
| dc.description.tableofcontents | 目錄…………………i 圖表目錄…………iii 中文摘要……………I ABSTRACT……………II 縮寫表………………III 第一章 緒論……………1 1.1 胞外間質帶金屬離子蛋白?家族的簡介…1 1.1.1 MMP蛋白的結構……………………2 1.2 明膠?的研究概況……………………3 1.2.1 明膠?的啟動子序列分析…………4 1.2.2 明膠?與胞外金屬?抑制蛋白……5 1.2.3 明膠?的活化……………………6 1.2.4 明膠?與荷爾蒙的調控………7 1.2.5 lipocalin與明膠?的關係……7 1.3 本論文的研究方向…………………8 第二章 實驗設計與方法………………15 2.1 實驗流程大綱……………………15 2.2 方法和材料……………………16 2.2.1 樣品的處理……………………16 2.2.2 雌鼠動情週期的判定……………………16 2.2.3 Total RNA的萃取……………………17 2.2.4 RNA品質的檢定……………………19 2.2.5 逆轉錄－聚合?連鎖反應(RTPCR)……21 2.2.6 聚合?連鎖反(PCR) …………………23 2.2.7 洋菜膠體泳……………………24 2.2.8 DNA片段萃取……………………25 2.2.9 DNA酒精沉澱……………………26 2.2.10 DNA接合反應……………………27 2.2.11 質體轉型……………………28 2.2.12 小量質體抽取…………………30 2.2.13 北方墨點法……………………31 2.2.14 組織蛋白萃取液……………35 2.2.15 蛋白質定量……………………36 2.2.16 SDS-聚丙烯醯胺膠體電泳………38 2.2.17 Serva Blue R染色法……………40 2.2.18 Zymography……………………41 2.2.19 西方墨點法……………………43 第三章 實驗結果……………………48 3.1 明膠?蛋白在雌鼠子宮動情週期的表現……48 3.1.1 Zymography……………48 3.1.2 西方墨點法……………………49 3.2 明膠?塞因在雌鼠子宮動情週期的表現……49 3.2.1 Gelatinase B cDNA的製備………49 3.2.2 北方墨點法……………………49 3.2.3 逆轉錄-聚合?連鎖反應……………50 3.3 明膠?和卵巢荷爾蒙調控的關係………50 第四章 討論…………………59 參考文獻……………………62 附錄一……………………66 附錄二……………………67 附錄三……………………69 附錄四……………………70 | |
| dc.language.iso | zh-TW | |
| dc.title | 明膠?在雌鼠子宮中動情週期的表現 | zh_TW |
| dc.title | The Expression of Gelatinase B in Mouse Uterus during Estrous Cycle | en |
| dc.date.schoolyear | 87-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 70 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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