HDA6和SUVH4/5/6 參與阿拉伯芥發育及逆境反應之功能性研究

Abstract

組蛋白去乙醯酶HDA6在基因調控與轉座子沉默扮演重要角色。SUVH4、SUVH5和SUVH6 (SUVH4/5/6)是組蛋白H3賴氨酸9(H3K9)甲基轉移酶，屬於SET結構域蛋白中SUV(R)亞群，也同樣是基因沉默的重要調控者。最近的研究發現SUVH4/5/6可以通過與HDA6互作改變轉座子的組蛋白甲基化和乙醯化狀態，調控轉座子沉默。然而，我們對於HDA6和SUVH4/5/6互作在植物生長發育和逆境調控的功能知之甚少。我們目前的研究發現 HDA6和SUVH4/5/6的四突變體hda6/suvh4/5/6植株有捲曲的葉形，也發現hda6/suvh4/5/6在高鹽濃度環境中發芽率明顯下降以及較短的根長度。由此，我們推斷HDA6和SUVH4/5/6可以共同調控葉片發育及非生物逆境反應。

HISTONE DEACETYLASE6 (HDA6) plays an important role in the silencing of transgenes and transposable elements. SUVH4, SUVH5, and SUVH6 (SUVH4/5/6) are histone H3 lysine 9 methyltransferases belonging to the SUV(R) group of SET domain proteins, which are also important in gene silencing. Recent studies show that SUVH4/5/6 and HDA6 interact and co-regulate the silencing of transposable elements by histone H3 deacetylation and H3 lysine 9 methylation. However, little is known about the functions of HDA6 and SUVH4/5/6 interaction in plant development and stress response. In this study, we found that the hda6/suvh4/5/6 quadruple mutant has obviously wrinkled and curling leaves. Furthermore, it also displays low seed germination rate and short root lengths under high concentration of NaCl compared to wild type. Together, these results indicate that HDA6 and SUVH4/5/6 may act coordinately in the regulation of leaf development and response to abiotic stress.