磷脂質輔助合成含鎂氫氧基磷灰石應用於骨修復材料

Abstract

含鎂氫氧基磷灰石應用於骨修復填補材料具有極大的發展潛力，但高鎂含量的氫氧基磷灰石之合成方法仍尚未發展成熟。在這項研究中，我們利用磷脂質(磷脂酰膽鹼)輔助合成含鎂氫氧基磷灰石，其中，鎂在材料中的重量百分比可控制在1.44至10.64 ％之間。利用磷脂質中帶負電荷的磷酸官能基可作用於陽離子(如鈣離子或鎂離子)，以提供含鎂氫氧基磷灰石穩定的成核。我們優化合成條件以獲得相對低結晶度的含鎂氫氧基磷灰石，透過X光繞射分析儀測量證明其晶相與人體骨骼相似。在體外試驗中，含鎂氫氧基磷灰石中的鎂離子釋放有利於骨細胞的增生和分化。綜上所述，這項研究闡述磷脂質應用於含鎂氫氧基磷灰石的新功效，並提供一種具前景的骨科生醫材料。

Magnesium-loaded hydroxyapatite (Mg@HAp) is a potential biomaterial for bone healing application but has not been comprehensively synthesized yet. In this study, we utilize a phosphatidylcholine (PC)-assisted method for synthesizing Mg@HAp with controllable Mg amount from 1.44 to 10.64 wt%. It is proposed that the negatively charged phosphate functional group of PC could act on cations (i.e. Mg2+ or Ca2+), which provides a stable nucleation for the formation of Mg@HAp. We optimize synthetic conditions for achieving a Mg@HAp with a relatively low crystallinity which is similar to human bone structure, as evidenced by XRD measurement. The in vitro test of bone cells (MG63) shows that the release of Mg2+ ions from Mg@HAp enhances cellular proliferation and differentiation. We believe the synthesized Mg@HAp would be a promising biomaterial in orthopedic applications.