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Chemical investigation of Dendrobium hybrids
Dendrobium hybrids,Dendrobium Taiseed Tosnobile,Dendrobium Cassiope,High performance liquid chromatography hyphenated solid phase extraction,flavones,
|Publication Year :||2018|
將八種石斛雜交種乙醇萃取物，以液-液分配法進行極性切割劃分為乙腈、乙酸乙酯、正丁醇和水可溶部分，再進一步對有機溶劑可溶部分進行成分分析。過程中使用Sephadex LH-20、分析及半製備級高效液相層析法、高效液相層析串聯固相萃取技術和質譜，共計鑑定出17個化合物，分別為2個苯甲酸衍生物 (benzoic acid derivatives, 1–2)、4個苯丙烷類 (phenylpropanoids, 3–6)、2個菲類 (phenanthrenes, 7–8) 及9個黃酮 (flavones, 9–17)，其中6、9–13、15–17共計9個化合物為首次自石斛屬植物發現。
Dendrobium (Shi Hu; Orchidaceae), which exhibits antioxidant, neuroprotective and immunomodulatory bioactivities, is a traditional Chinese medicine. Because of the growth of the wild type Dendrobium species is normally slow, hybridization of the fast-growing species with the rare and valuable ones is a feasible strategy to overcome the difficulties. Thus the aim of this research was to investigate the chemical constituents of the Dendrobium hybrids and to compare their differences in the chemical constituents.
The ethanolic extracts of the eight hybrids were divided into fractions soluble in ACN, EtOAc, n-BuOH, and water via liquid-liquid partitioning. Further separation was conducted on the organic solvent soluble fractions. This effort led to the identification of 17 compounds by combination of Sephadex LH-20, analytical and semi-preparative reverse phase high performance liquid chromatography (RP-HPLC), HPLC-DAD-SPE (solid phase extraction) and MS. These compounds included benzoic acid derivatives (1–2), phenylpropanoids (3–6), phenanthrenes (7–8) and flavones (9–17). Of these, nine compounds, i.e., 6, 9–13, 15–17, were new to the Dendrobium species. Their structures were elucidated by MS data and NMR spectropic analysis.
Phenylpropanoids were the major compound isolated from Dendrobium Taiseed Tosnobile. Flavones could be identified as the major compounds in other Dendrobium hybrids. The amount of compounds 12, 14 and 17 in (♀) Dendrobium tosaense × (♂) Dendrobium linawianum is 2.2 to 3.3 times more than that in (♀) Dendrobium nobile × (♂) Dendrobium linawianum and in (♀) Dendrobium linawianum × (♂) Dendrobium nobile. The amount of compouds 9 and 10 in (♀) Dendrobium tosaense × (♂) Dendrobium huoshanense is 2.8 to 5 times more than that in Dendrobium Taiseed Tosnobile and (♀) Dendrobium tosaense × (♂) Dendrobium nobile. Another, the amount of compounds 13 and 15 in (♀) Dendrobium moniliforme × (♂) Dendrobium nobile is 1.3 times more than that in Dendrobium Cassiope. However, the amount of compound 16 in Dendrobium Cassiope is 1.6 times more than that in (♀) Dendrobium moniliforme × (♂) Dendrobium nobile. This research concluded the high polarity compounds of the eight Dendrobium hybrids, especially the amount of phenylpropanoids and C-glycosyl flavones, and the conclusion could be the reference for the following development of the titled medicine.
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