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標題: | 石斛雜交種化學成分之研究 Chemical investigation of Dendrobium hybrids |
作者: | 林士翔 Shih-Hsiang Lin |
指導教授: | 張嘉銓 Chia-Chuan Chang |
關鍵字: | 石斛雜交種,金皇石斛,金童石斛,高效液相層析串聯固相萃取技術,黃酮化合物, Dendrobium hybrids,Dendrobium Taiseed Tosnobile,Dendrobium Cassiope,High performance liquid chromatography hyphenated solid phase extraction,flavones, |
出版年 : | 2018 |
學位: | 碩士 |
摘要: | 蘭科石斛為一種傳統中國藥材,具有抗氧化、神經保護及免疫調節等作用。由於野生種石斛生長緩慢,因此,將具有生長快速之特性的品種與有效成分含量高的品種雜交,培養出的品種可能具有生長快速且有效成分含量高。為了比較其差異,本研究旨於分析石斛雜交種化學成分。
將八種石斛雜交種乙醇萃取物,以液-液分配法進行極性切割劃分為乙腈、乙酸乙酯、正丁醇和水可溶部分,再進一步對有機溶劑可溶部分進行成分分析。過程中使用Sephadex LH-20、分析及半製備級高效液相層析法、高效液相層析串聯固相萃取技術和質譜,共計鑑定出17個化合物,分別為2個苯甲酸衍生物 (benzoic acid derivatives, 1–2)、4個苯丙烷類 (phenylpropanoids, 3–6)、2個菲類 (phenanthrenes, 7–8) 及9個黃酮 (flavones, 9–17),其中6、9–13、15–17共計9個化合物為首次自石斛屬植物發現。 金皇石斛可分得最多含量之苯丙烷類,其餘石斛雜交種可分得含量多之黃酮化合物:化合物12、14、17在黃櫻石斛中之含量比其在金櫻石斛及櫻金石斛中多出2.2–3.3倍,黃米石斛中所含之9及10高於金皇及黃金石斛所含之2.8–5.0倍;另外,銅金石斛所含之13及15較金童石斛之含量高出1.3倍,然而金童石斛所含之16較銅金石斛之含量高出1.6倍。本次研究總結八種石斛雜交種之高極性化學成分,特別是苯丙烷及黃酮碳苷化合物之含量差異,可作為後續藥材開發之參考。 Dendrobium (Shi Hu; Orchidaceae), which exhibits antioxidant, neuroprotective and immunomodulatory bioactivities, is a traditional Chinese medicine. Because of the growth of the wild type Dendrobium species is normally slow, hybridization of the fast-growing species with the rare and valuable ones is a feasible strategy to overcome the difficulties. Thus the aim of this research was to investigate the chemical constituents of the Dendrobium hybrids and to compare their differences in the chemical constituents. The ethanolic extracts of the eight hybrids were divided into fractions soluble in ACN, EtOAc, n-BuOH, and water via liquid-liquid partitioning. Further separation was conducted on the organic solvent soluble fractions. This effort led to the identification of 17 compounds by combination of Sephadex LH-20, analytical and semi-preparative reverse phase high performance liquid chromatography (RP-HPLC), HPLC-DAD-SPE (solid phase extraction) and MS. These compounds included benzoic acid derivatives (1–2), phenylpropanoids (3–6), phenanthrenes (7–8) and flavones (9–17). Of these, nine compounds, i.e., 6, 9–13, 15–17, were new to the Dendrobium species. Their structures were elucidated by MS data and NMR spectropic analysis. Phenylpropanoids were the major compound isolated from Dendrobium Taiseed Tosnobile. Flavones could be identified as the major compounds in other Dendrobium hybrids. The amount of compounds 12, 14 and 17 in (♀) Dendrobium tosaense × (♂) Dendrobium linawianum is 2.2 to 3.3 times more than that in (♀) Dendrobium nobile × (♂) Dendrobium linawianum and in (♀) Dendrobium linawianum × (♂) Dendrobium nobile. The amount of compouds 9 and 10 in (♀) Dendrobium tosaense × (♂) Dendrobium huoshanense is 2.8 to 5 times more than that in Dendrobium Taiseed Tosnobile and (♀) Dendrobium tosaense × (♂) Dendrobium nobile. Another, the amount of compounds 13 and 15 in (♀) Dendrobium moniliforme × (♂) Dendrobium nobile is 1.3 times more than that in Dendrobium Cassiope. However, the amount of compound 16 in Dendrobium Cassiope is 1.6 times more than that in (♀) Dendrobium moniliforme × (♂) Dendrobium nobile. This research concluded the high polarity compounds of the eight Dendrobium hybrids, especially the amount of phenylpropanoids and C-glycosyl flavones, and the conclusion could be the reference for the following development of the titled medicine. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/69889 |
DOI: | 10.6342/NTU201800602 |
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顯示於系所單位: | 藥學系 |
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