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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 郭錦樺(Ching-hua Kuo) | |
| dc.contributor.author | Wei-Qing Yang | en |
| dc.contributor.author | 楊崴清 | zh_TW |
| dc.date.accessioned | 2021-06-17T00:33:43Z | - |
| dc.date.available | 2014-03-02 | |
| dc.date.copyright | 2012-03-02 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-02-09 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/66395 | - |
| dc.description.abstract | 癌症現在仍是世界首要死因。近年來在質譜和核磁共振儀器技術上的進步加上化學計量學讓代謝體在癌症研究上有更多應用。本論文含兩部分，第一部分用極致超高壓液相層析儀結合飛行式質譜儀 (UHPLC-TOF MS) 找尋和乳癌發展相關的代謝物。第二部分利用頂空固相微萃取結合氣相層析飛行式質譜儀 (HS-SPME GC-TOFMS) 方法分析人體呼出氣體濃縮液 (EBC) 。最終目的是找到可用作肺癌臨床診斷的代謝生物性指標。
在第一部分實驗中，我們用UHPLC-TOF MS 分析三十八位不同乳癌分級已停經女性乳癌患者的血液樣品。並用Kruskal-Wallis rank sum test 找到在不同乳癌分級的分組有明顯差異的代謝物。其中我們找到十四個在不同乳癌分級的分組有明顯差異的代謝物(p<0.05)，其中11個代謝物表現出和乳癌分級有相關趨勢。這些代謝物和choline phospholipid 代謝, sphingolipid 代謝, polyamine 生合成和氧化還原途徑相關。論文中對這些代謝物在癌症發展中扮演的角色進行討論。 在第二部分開發HS-SPME GC-TOFMS 方法來偵測人體EBC中的分子。最佳化條件如下：200 毫克氯化鈉加入0.5毫升的EBC，PDMS/DVB纖維插入樣品瓶，在45℃下頂空萃取四小時。再到氣相層析儀注入口，不分流情況下，在250℃下熱脫附三分鐘。此方法被應用在分析健康人和肺癌病患的EBC樣品。結果顯示1-ethynyl-1-cyclohexanol 在大多數的肺癌病患的EBC中被偵測到，但在健康人的EBC中沒偵測到。另一方面 1-dodecanol, (E)-5-octadecene, butyl acetate, 1,3-bis(1,1-dimethylethyl)-benzene, and 3-methyl-pentane在肺癌病患中是沒被偵測到或強度很低。 初步實驗結果指出質譜代謝體可被利用來研究乳癌和肺癌。進一步的研究需要擴充樣品數量來對結果進行驗證。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-06-17T00:33:43Z (GMT). No. of bitstreams: 1 ntu-101-R98423031-1.pdf: 1045170 bytes, checksum: 00d9996b0748ba93a4889fc0fd9dddcc (MD5) Previous issue date: 2012 | en |
| dc.description.tableofcontents | Contents I
Abstract in Chinese III Abstract IV Preface 1 Metabolomics 1 Application of Mass Spectrometry-Based Metabolomics in Cancer Research 4 Part I: Metabolic Profiling of Breast Cancer by Ultra High Pressure Liquid Chromatography Time-of-Flight Mass Spectrometry 6 Section I: Introduction 7 Section II: Material and Method 12 Section III: Result 16 Section IV: Discussion 19 Section V: Conclusion 26 Section VI: Reference 27 Section VII: Figures and Tables 44 Figure 1. Total ion chromatograms obtained from serum samples of patients with different grades 44 Figure 2. Principal component analysis (PCA) of the differential features among different tumor grade groups 45 Figure 3. Boxplots of eleven significant metabolites in breast cancer patients with different tumor grades 46 Figure 4. A network of differential metabolites related to tumor grade 47 Table 1. Patient characteristics 48 Table 2. Serum metabolites that show significantly different intensities between breast cancer patients with different grades 49 Part II: Development of a Headspace Solid-Phase Microextraction Combined With Gas Chromatography Time-of-Flight Mass Spectrometry Method for Profiling Exhaled Breath Condensate Volatile Metabolic Patterns 50 Section I: Introduction 51 Section II: Material and Method 56 Section III: Result and Discussion 60 Section IV: Conclusion 68 Section V: Reference 69 Section VI: Figures and Tables 77 Figure 1. Venn diagrams of compounds distribution among three replicate injections by DVBCARPDMS or PDMSDVB fibers 77 Figure 2. The number of peaks comparison between two extraction temperatures 77 Figure 3. The number of peaks comparison between two EBC sample volumes 78 Figure 4. The number of peaks comparison among three desorption times 78 Figure 5. The number of peaks comparison between EBC samples with and without NaCl added 79 Figure 6. Average number of peaks detected with different extraction time 79 Figure 7. The representative metabolic profiles of lung cancer patients and healthy subjects 80 Table 1. The characteristics of lung cancer patients and healthy subjects 80 | |
| dc.language.iso | en | |
| dc.subject | 呼出氣體濃縮液 | zh_TW |
| dc.subject | 代謝體學 | zh_TW |
| dc.subject | 肺癌 | zh_TW |
| dc.subject | 乳癌 | zh_TW |
| dc.subject | 質譜儀 | zh_TW |
| dc.subject | breast cancer | en |
| dc.subject | Metabolomics | en |
| dc.subject | exhaled breath condensate | en |
| dc.subject | lung cancer | en |
| dc.subject | mass spectrometry | en |
| dc.title | 質譜儀在癌症代謝體的應用
第一部分：利用極致超高壓液相層析儀結合飛行式質譜儀分析乳癌患者的代謝圖譜 第二部分：開發頂空固相微萃取結合氣相層析儀飛行式質譜儀方法分析呼出氣體濃縮液的代謝圖譜 | zh_TW |
| dc.title | Application of Mass Spectrometry-Based Metabolomics in Cancer Research
Part I: Metabolic Profiling of Breast Cancer by Ultra High Pressure Liquid Chromatography Time-of-Flight Mass Spectrometry Part II: Development of a Headspace Solid-Phase Microextraction Combined With Gas Chromatography Time-of-Flight Mass Spectrometry Method for Profiling Exhaled Breath Condensate Volatile Metabolic Patterns | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 曾宇鳳(Y. Jane Tseng),蔡詩偉(Shih-Wei),黃俊升(Chiun-Sheng Huang) | |
| dc.subject.keyword | 代謝體學,肺癌,乳癌,質譜儀,呼出氣體濃縮液, | zh_TW |
| dc.subject.keyword | Metabolomics,lung cancer,breast cancer,mass spectrometry,exhaled breath condensate, | en |
| dc.relation.page | 80 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2012-02-09 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 藥學研究所 | zh_TW |
| 顯示於系所單位： | 藥學系 | |
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