以電紡絲法製備幾丁聚醣/聚乙烯醇同軸奈米纖維及其應用

Abstract

本研究針對以電紡絲法製備幾丁聚醣與聚乙烯醇的同軸奈米纖維進行探討。由於幾丁聚醣為帶有正電的高分子材料，在一般電紡絲法中難以形成連續的奈米纖維，而同軸電紡法正好可以改善這個缺點。過程中針對不同的電紡參數進行測試，藉由改變溶液濃度、流速、溶劑中醋酸的比例、核殼層溶液選擇，觀察其對電紡結果的影響。接著選出幾組奈米纖維型態較佳的組別，進行一連串的性質測定。另外，使用戊二醛對奈米纖維進行交聯以固定纖維型態。最後將其應用於藥物釋放，期許能有穩定的釋放曲線。 本實驗成功製備出具有同軸結構的奈米纖維，其核層為聚乙烯醇，殼層為幾丁聚醣。由於殼層部分是兼具生物相容性與生物降解性的幾丁聚醣，若再搭配藥物穩定釋放的特性(其中藥物可選用幫助細胞增殖分化的生長因子等)，相信在細胞培養以及組織工程應用上會有更廣泛的發展空間，因此是極具潛力的生醫材料。

In this research, coaxial electrospinning method was used to prepare chitosan and polyvinyl alcohol (PVA) nanofibers with a core-shell structure. Chitosan is a cationic biopolymer that is challenging to electrospin, but this difficulty could be overcome by using coaxial electrospinning. Various solutions with different concentrations and compositions of chitosan and PVA were prepared. The solution concentrations and flow rates in core and shell layers were varied to observe the results of coaxial electrospinning. Nanofibers with best morphology were chosen for further analyses and crosslinked using glutaraldehyde to improve their mechanical strength. In the application of the nanofibers in drug delivery, a sustained release of drugs was expected. The core/shell (PVA/chitosan) structured nanofibers were successfully obtained in this research. In addition, the shell layer was made up of chitosan which has good biocompatibility and biodegradability. With the sustained release of drugs (such as growth factors which favor cell proliferation and differentiation), it is believed that the prepared coaxial nanofibers can be used as biomaterials and have potential in cell culture and tissue engineering-related applications.