人類CD8+CD127hi及CD127lo的TEM與TEMRA次群的分化及T細胞受體庫分析

Abstract

Human CD8+ T cell can be separate into naive, central memory, effector memory (TEM) and terminally differentiated effector cells (TEMRA), based on the expression of CD45RA and CCR7. In our previous study, we found that, a higher frequency of CD127loTEM and TEMRA among total CD8+T cells was found in peripheral blood or tumor infiltrating lymphocytes, but not in regional lymph nodes of oral cancer patients. The CD127hi TEM and TEMRA cells maintained higher IFN-γ, IL-2 production and ex vivo proliferative capacity, while the CD127lo TEM and TEMRA cells exhibited higher granzyme B productivity and susceptibility to activation induced cell death. Therefore, CD127 hi TEM and TEMRA cells exhibit memory T cell phenotype, while CD127lo TEM and TEMRA cells exhibit late differentiated effector phenotype. The specific aim of my study is to estimate the extent of Vβ TCR diversity and to exam the clonally expanded T cell populations among these 4 distinct CD8+T cell subsets using high-throughput DNA sequencing. There is increased frequency of CD127loTEM and TEMRACD8+ subsets in patient with OSCC and OLP. The frequency of peripheral CD127loTEM in T3 and T4 patients is higher than T1 and T2 patients. After ex vivo expansion with anti-CD3/CD28 beads, all the T cell populations exhibit the CD127loCD45RA- phenotype.