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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 王萬波 | |
dc.contributor.author | Hui-Yi Lin | en |
dc.contributor.author | 林惠怡 | zh_TW |
dc.date.accessioned | 2021-06-16T05:09:58Z | - |
dc.date.available | 2019-10-09 | |
dc.date.copyright | 2014-10-09 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-08-19 | |
dc.identifier.citation | Bachmann, M., and Moroy, T. (2005). The serine/threonine kinase Pim-1. Int J Biochem Cell Biol. 37, 726-730.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/55876 | - |
dc.description.abstract | Human CD8+ T cell can be separate into naive, central memory, effector memory (TEM) and terminally differentiated effector cells (TEMRA), based on the expression of CD45RA and CCR7. In our previous study, we found that, a higher frequency of CD127loTEM and TEMRA among total CD8+T cells was found in peripheral blood or tumor infiltrating lymphocytes, but not in regional lymph nodes of oral cancer patients. The CD127hi TEM and TEMRA cells maintained higher IFN-γ, IL-2 production and ex vivo proliferative capacity, while the CD127lo TEM and TEMRA cells exhibited higher granzyme B productivity and susceptibility to activation induced cell death. Therefore, CD127 hi TEM and TEMRA cells exhibit memory T cell phenotype, while CD127lo TEM and TEMRA cells exhibit late differentiated effector phenotype. The specific aim of my study is to estimate the extent of Vβ TCR diversity and to exam the clonally expanded T cell populations among these 4 distinct CD8+T cell subsets using high-throughput DNA sequencing. There is increased frequency of CD127loTEM and TEMRACD8+ subsets in patient with OSCC and OLP. The frequency of peripheral CD127loTEM in T3 and T4 patients is higher than T1 and T2 patients. After ex vivo expansion with anti-CD3/CD28 beads, all the T cell populations exhibit the CD127loCD45RA- phenotype. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T05:09:58Z (GMT). No. of bitstreams: 1 ntu-103-R01445117-1.pdf: 3311281 bytes, checksum: ba44fb3abb30378740ea81988cabef77 (MD5) Previous issue date: 2014 | en |
dc.description.tableofcontents | 口試委員審定書 ii
致謝 iii 中文摘要 iv Abstract v Chapter 1. Introduction 1 1.1. Memory CD8+T Cell heterogeneity 1 1.2. Effector and memory CD8+ Cell differentiation 3 1.3. IL-7 receptor (CD127) in CD8 memory T cells 4 1.4. TCR repertoire 6 1.5. Oral cancer 7 1.6. Oral lichen planus 7 Chapter 2. Purposes and Aims 9 Chapter 3. Materials and Methods 10 3.1. Human Subjects 16 3.2. Peripheral blood mononuclear cells (PBMCs) and CD8+ T cells Isolation 16 3.3. Isolation of tumor-infiltrating lymphocytes (TILs) 17 3.4. Flow cytometry analysis (FACS) 17 3.5. Cell sorting 18 3.6. Message RNA extraction and real-time quantitative PCR 18 3.7. Cell proliferation 19 3.8. TCR repertoire sample preparation and analysis 20 3.9. Statistical analysis 21 Chapter 4. Results 22 4.1. Increasing of CD127loTEM and TEMRA CD8+T cell subsets in patient with OSCC and OLP 22 4.2. Correlation of CD127lo TEM and TEMRA CD8+T cell subsets in disease progression. 23 4.3. CD8+T cell differentiation associated gene expression in peripheral CD127hi or CD127lo TEM and TEMRA 24 4.4. Expansion and differentiation potential of human CD8+T cell subsets 25 4.5. TCR Repertoire analysis of Human CD8+ CD127hi or CD127lo TEM and TEMRA 27 Chapter 5. Discussion 29 Chapter 6. Reference 32 Chapter 7. Tables 38 Chapter 8. Figures 43 | |
dc.language.iso | en | |
dc.title | 人類CD8+CD127hi及CD127lo的TEM與TEMRA次群的分化及T細胞受體庫分析 | zh_TW |
dc.title | Differentiation and TCR Repertoire Analysis of Human CD8+CD127hi or CD127loTEM and TEMRA | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 賈景山 | |
dc.contributor.oralexamcommittee | 賴明宗,許秉寧 | |
dc.subject.keyword | IL-7受體,口腔癌, | zh_TW |
dc.subject.keyword | CD127,OSCC, | en |
dc.relation.page | 60 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2014-08-19 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 微生物學研究所 | zh_TW |
顯示於系所單位: | 微生物學科所 |
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