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Title: | 可3D列印的奈米黏土水凝膠於生醫骨架的應用 3D Printable Nanoclay Based Hydrogel for Biomedical Scaffold |
Authors: | Wei-Ting Ke 柯威廷 |
Advisor: | 廖英志(Ying-Chih Liao) |
Keyword: | 3D生物列印,奈米黏土,交聯機制,水凝膠,生醫骨架, 3D bioprinting,nanoclay,crosslinking mechanism,hydrogel,biomedical scaffold, |
Publication Year : | 2020 |
Degree: | 碩士 |
Abstract: | 直寫式3D生物列印技術,於近年來常被應用於組織工程領域,透過擠出成型的方式將生物材料塗佈於基材,以製備三維結構的生醫骨架,得以用於複雜立體結構的組織與器官再生,其中水凝膠因具備高度生物相容性、生物可分解、高孔隙度等特性,經常被選作生物墨水的主要成分,並結合生物列印技術而產生多種應用。然而將水凝膠應用於3D列印,必須克服水凝膠流動性太強、與支撐力薄弱等問題,此外所印製出的結構,也必須具備高精度、抗膨潤、高機械強度、高細胞活性等特性,才能提供細胞貼附生長所需的支撐力,並維持形狀。常見的生物型水凝膠,包含海藻酸、膠原蛋白、玻尿酸、明膠等等,往往欠缺足夠的屈伏應力而無法順利成形固化,另外若透過化學交聯或後處理的方式提升機械性質,則往往犧牲凝膠的可印製性,並有可能影響細胞活性。 本研究則提出一種新型可3D列印的水凝膠製備方法,我們選用奈米黏土laponite作為主要原料,並提出其與醛基化奈米纖維素微晶的化學交聯機制,為了探討此種化學交聯對凝膠可印製特性的影響,我們調控醛基化奈米纖維素與奈米黏土的比例,探討不同比例下對凝膠流變性質、可印製性、結構精度、機械強度與膨潤特性等影響,實驗結果可知在6%奈米黏土與1%醛基化奈米纖維素的比例下,具最佳的可印製性與結構精度,另外可提升約4倍的機械強度與降低約90%的膨潤現象。此種水凝膠省去前人印製水凝膠所需的後處理步驟,印製成品亦具備優良的結構穩定性與生物活性,可成功印製複雜立體結構。簡言之,本研究提出一種新型可印製水凝膠,並為3D生物列印技術拓展一條嶄新的道路。 In recent years, direct-write 3D bioprinting has been used in the field of tissue engineering. Biomaterials are printed on substrates by extrusion method to fabricate three-dimensional biomedical scaffold, which can be applied in the regeneration of organs or tissues with complex structure. Among all biomaterials, hydrogel is usually chosen as the main component of bio-ink due to the high biocompatibility, biodegradability, high porosity and so forth. The combination between hydrogel and bioprinting technology is then generating various of applications. However, high fluidity and low self-supporting ability hinder the use of hydrogel in 3D printing. Moreover, the printed structure should possess high fidelity, anti-swelling behavior, high mechanical strength, high cell viability and so forth in order to provide supporting for cell adhesion and growth, while maintaining its shape. Commonly seen biogels, such as alginate, collagen, Hyaluronic acid, gelatin and so forth, are hard to solidify due to the lack of sufficient yield stress. However, increasing mechanical strength by chemical crosslinking or post-treatment sacrifices the printability of hydrogel and might influence the cell viability. In this thesis, a novel synthesis method is developed to formulate 3D printable hydrogel. We choose nanoclay, laponite, as the main component and propose a mechanism of crosslinking laponite and dialdehyde cellulose nanocrystal (DAC). To evaluate the influence of the chemical crosslinking on the printability of hydrogel, we adjust the ratio of laponite and DAC and analyze the influence of different compositions on rheology, printability, fidelity, mechanical strength, swelling behavior and so forth. The results show that the composition of 6% of laponite and 1% of DAC possesses the best printability and fidelity. Moreover, the mechanical strength is enhanced by 4 times and the swelling behavior is decreased by 90%. This method omits the complicated post-treatment that is essential for other printable hydrogels. The final printed structure possesses excellent structural stability and cell viability. The complicated hydrogel structure is then achieved. In summary, we propose a new printable hydrogel and pave the way for the development of 3D bioprinting technology. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/54417 |
DOI: | 10.6342/NTU202002276 |
Fulltext Rights: | 有償授權 |
Appears in Collections: | 化學工程學系 |
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U0001-0308202015031900.pdf Restricted Access | 5.17 MB | Adobe PDF |
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