請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/52225
標題: | 戊二醛改質之環狀微流道用於捕捉兩種T型淋巴細胞之
研究 Capturing Two Types of T Lymphocyte with Circular Microfluidics Functionalized by Glutaraldehyde |
作者: | Yeu-Farn Shih 施禹帆 |
指導教授: | 李世光(Chih-Kung Lee) |
關鍵字: | 微流道,細胞捕捉, microfluidics,cell capture, |
出版年 : | 2015 |
學位: | 碩士 |
摘要: | 目前世界上有三分之ㄧ人口可能感染肺結核,潛伏期肺結核患者不會有病狀,但在未來幾年有5%-10%機率會發病,尤其在受感染當年機會最高,雖然目前有一種新的檢測方法-丙型干擾素檢測方法(Interferon Gamma Release Assay, IGRA),可以藉由量測T型細胞因受到刺激而產生的丙型干擾素診斷肺結核症狀,然而,此種方法並無法知道有多少數量的T細胞分泌丙型干擾素,會降低最後結果的精準度,因此本研究發展一個以戊二醛改質的環狀微流道捕捉T型細胞,透過有限元素軟體COMSOL模擬以及最佳化微流道裡的微柱子陣列,使得流道內的流場可以均勻分布,實驗結果也證明環狀微流道在旋轉角30˚的情況下有最好的捕捉效率,之後,選擇兩種T型細胞(CD4細胞與CD8細胞)測試抗體的專ㄧ性結合,配合實驗參數的重新設定,像是化學溶液的濃度、反應時間、流速,成功以環狀微流道鍵結特定的抗體捕捉兩種T型淋巴細胞。 It is estimated that about one-third of the world’s population has already been infected by tuberculosis. Mycobacterium tuberculosis, in general, can result in an active case of tuberculosis in approximately 5%-10% of those who suffer from latent tuberculosis and the chance of becoming ill is the highest within one of year of getting the disease. Although a newly developed methods called interferon gamma release assay (IGRA) can monitor T cells secreted cytokine to diagnose tuberculosis (TB) condition. However, it is difficult to count total numbers of cytokine secreted T cells, which make the diagnosis less accurate. Therefore, this paper develops a functionalized polydimethylsiloxane (PDMS) device using glutaraldehyde to capture T cells. To enhance the capture efficiency, we use COMSOL simulation to optimize the arrangement of PDMS micro pillars to make cells uniformly distributed in the device. The preliminary data showed the microfluidic configuration in a circular shape with HCP patterned micro pillars turned 30 degrees offers the highest cell capture rate. Afterwards, we choose two types of T lymphocyte (CD4+T cell and CD8+T cell) for testing the specificity between antibody and antigen around the cells. By resetting the parameters like chemicals’ concentration, reaction time and flow velocity, we successfully capture different cells with specific antibody in circular microfluidics. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/52225 |
全文授權: | 有償授權 |
顯示於系所單位: | 工程科學及海洋工程學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-104-1.pdf 目前未授權公開取用 | 4.91 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。