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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 李世光(Chih-Kung Lee) | |
dc.contributor.author | Yeu-Farn Shih | en |
dc.contributor.author | 施禹帆 | zh_TW |
dc.date.accessioned | 2021-06-15T16:09:51Z | - |
dc.date.available | 2018-08-25 | |
dc.date.copyright | 2015-08-25 | |
dc.date.issued | 2015 | |
dc.date.submitted | 2015-08-18 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/52225 | - |
dc.description.abstract | 目前世界上有三分之ㄧ人口可能感染肺結核,潛伏期肺結核患者不會有病狀,但在未來幾年有5%-10%機率會發病,尤其在受感染當年機會最高,雖然目前有一種新的檢測方法-丙型干擾素檢測方法(Interferon Gamma Release Assay, IGRA),可以藉由量測T型細胞因受到刺激而產生的丙型干擾素診斷肺結核症狀,然而,此種方法並無法知道有多少數量的T細胞分泌丙型干擾素,會降低最後結果的精準度,因此本研究發展一個以戊二醛改質的環狀微流道捕捉T型細胞,透過有限元素軟體COMSOL模擬以及最佳化微流道裡的微柱子陣列,使得流道內的流場可以均勻分布,實驗結果也證明環狀微流道在旋轉角30˚的情況下有最好的捕捉效率,之後,選擇兩種T型細胞(CD4細胞與CD8細胞)測試抗體的專ㄧ性結合,配合實驗參數的重新設定,像是化學溶液的濃度、反應時間、流速,成功以環狀微流道鍵結特定的抗體捕捉兩種T型淋巴細胞。 | zh_TW |
dc.description.abstract | It is estimated that about one-third of the world’s population has already been infected by tuberculosis. Mycobacterium tuberculosis, in general, can result in an active case of tuberculosis in approximately 5%-10% of those who suffer from latent tuberculosis and the chance of becoming ill is the highest within one of year of getting the disease. Although a newly developed methods called interferon gamma release assay (IGRA) can monitor T cells secreted cytokine to diagnose tuberculosis (TB) condition. However, it is difficult to count total numbers of cytokine secreted T cells, which make the diagnosis less accurate. Therefore, this paper develops a functionalized polydimethylsiloxane (PDMS) device using glutaraldehyde to capture T cells. To enhance the capture efficiency, we use COMSOL simulation to optimize the arrangement of PDMS micro pillars to make cells uniformly distributed in the device. The preliminary data showed the microfluidic configuration in a circular shape with HCP patterned micro pillars turned 30 degrees offers the highest cell capture rate. Afterwards, we choose two types of T lymphocyte (CD4+T cell and CD8+T cell) for testing the specificity between antibody and antigen around the cells. By resetting the parameters like chemicals’ concentration, reaction time and flow velocity, we successfully capture different cells with specific antibody in circular microfluidics. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T16:09:51Z (GMT). No. of bitstreams: 1 ntu-104-R02525030-1.pdf: 5027880 bytes, checksum: c3887a0c259094aad0eb3d06b6d09693 (MD5) Previous issue date: 2015 | en |
dc.description.tableofcontents | 口試委員會審定書 #
致 謝 i 中文摘要 ii ABSTRACT iii 目錄 iv 圖目錄 vii 表目錄 xi 第1章 緒論 1 1.1 研究背景及動機 1 1.2 文獻回顧 3 1.2.1 酵素免疫分析法與流式細胞儀 3 1.2.2 微流道捕捉細胞 5 1.2.3 依外力捕捉細胞 8 1.2.4 不依外力捕捉細胞 12 1.3 論文架構 15 第2章 實驗原理 16 2.1 有限元素軟體模擬分析 16 2.1.1 流道繪圖設計 16 2.1.2 有限元素軟體分析流場 18 2.1.3 有限元素軟體分析粒子行進軌跡 23 2.2 流道表面改質原理 25 2.2.1 氧化PDMS表面 26 2.2.2 表面官能化 26 2.2.3 戊二醛作為連接分子 27 2.2.4 結合抗體 27 2.2.5 防止非專ㄧ性鍵結 28 2.2.6 抗體捕捉細胞機制 29 第3章 實驗流程 30 3.1 微流道製程 30 3.1.1 矽晶圓微流道模具製作 31 3.1.2 微流道製作 36 3.1.3 流道封裝 38 3.2 細胞培養 39 3.3 流道改質過程 41 3.3.1 抗體與化學藥品 41 3.3.2 實驗流程 42 3.4 細胞觀測 44 3.4.1 細胞染色 44 3.4.2 計算細胞數量 44 3.4.3 細胞觀測 45 3.4.4 計算捕捉細胞數量 47 第4章 實驗結果分析與討論 50 4.1 環狀微流道 50 4.2 螢光珠模擬細胞行進軌跡 52 4.3 抗體捕捉細胞 56 4.4 捕捉兩種不同T型細胞 60 4.4.1 相關參數調整 60 4.4.2 環狀微流道鍵結CD4抗體捕捉HH細胞專ㄧ性測試 63 4.4.3 環狀微流道鍵結CD8抗體捕捉TALL-104細胞專ㄧ性測試 66 4.4.4 微柱子捕捉細胞 68 第5章 結論與未來展望 70 5.1 結論 70 5.2 未來展望 71 參考文獻 72 | |
dc.language.iso | zh-TW | |
dc.title | 戊二醛改質之環狀微流道用於捕捉兩種T型淋巴細胞之
研究 | zh_TW |
dc.title | Capturing Two Types of T Lymphocyte with Circular Microfluidics Functionalized by Glutaraldehyde | en |
dc.type | Thesis | |
dc.date.schoolyear | 103-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 黃念祖(Nien-Tsu Huang) | |
dc.contributor.oralexamcommittee | 吳文中(Wen-Jong Wu),李舒昇(Shu-Sheng Lee),陳奕帆(Yih-Fan Chen) | |
dc.subject.keyword | 微流道,細胞捕捉, | zh_TW |
dc.subject.keyword | microfluidics,cell capture, | en |
dc.relation.page | 74 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2015-08-19 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 工程科學及海洋工程學研究所 | zh_TW |
顯示於系所單位: | 工程科學及海洋工程學系 |
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