Lactobacillus paracasei subsp. paracasei NTU 101 菌粉對於 loperamide 誘導便秘大鼠之改善效果評估

Abstract

便秘為常見之腸胃道症狀，飲食及生活習慣改變或代謝、神經系統等方面疾病會導致便秘，便秘也會使腸道老化，其亦為大腸癌之風險因子之一。目前已有許多文獻指出乳酸菌可增加便秘患者之排便頻率及糞便含水量。本研究以 Lactobacillus paracasei subsp. paracasei NTU 101 原末菌粉評估便秘之改善功效。以每日 2 次皮下注射 loperamide (2 mg/kg BW) 減緩 Sparque-Dawley (SD) 大鼠腸道蠕動速率誘導便秘產生，同時介入低、中、高劑量之 NTU 101 原末菌粉 (1.3、2.6、13.0 mg/kg BW) 探討其改善效果。結果顯示管餵 NTU 101 菌粉低、中、高劑量均可增加腸道蠕動速率，分別較誘導組上升 12.4%、13.2% 與 15.0%；並可增加糞便含水量，分別較誘導組增加7.6%、8.4% 與 10.9%，此結果顯示低劑量即有改善排便之功效，且隨著劑量增加效果更佳。此外，餵食 NTU 101 原末菌粉可增加糞便中Bifidobactrium spp. 菌數，減少Clostridium perfringens 菌數，增加糞便短鏈脂肪酸濃度及降低 pH 值，並能夠增加結腸黏膜層厚度 (mucosal thickness)、分泌黏液之杯狀細胞 (goblet cells) 及卡哈氏間質細胞 (interstitial cells of Cajal, ICC)。綜合以上結果，NTU 101 原末菌粉可改善由 loperamide 所引起之便秘症狀外，也具有改善腸胃道功能之效果。

Constipation is a common gastrointestinal symptom. Life style, eating habit, metabolic conditions and neurological disorders could cause the constipation which is also one of the risk factors of colorectal cancer. Lactic acid bacteria have been reported to improve defecation frequency and fecal water content so far. This study evaluates the effect of Lactobacillus paracasei subsp. paracasei NTU 101 on constipation. Sparque-Dawley (SD) rats were subcutaneously injected loperamide (2 mg/kg BW) twice a day with 1.3, 2.6, and 13.0 mg/kg BW/rat/day of NTU 101 powder, respectively. As a result, three doses of NTU 101 powder improved 12.4%, 13.2%, and 15.0% of intestinal motility, respectively, compared with loperamide group. It also improved 7.6%, 8.4%, and 10.9% of fecal water content, respectively, compared with loperamide group. This result shows that low dosage has the effect of improving defecation, and that has the better effect with dose ascension. In addition, NTU 101 powder increased Bifidobacterium spp. and decreased Clostridium perfringens contents in feces. It also enhanced concentration of short chain fatty acids and reduced pH value in feces. Moreover, it could promote colonic mucosal thickness, mucin producing-goblet cell, and interstitial cells of Cajal (ICC). Based on the above results, NTU 101 powder can not only improve the constipation induced by loperamide, but also improve the function of gastrointestinal tract.