次微米級可程式電致蛋白質塗佈技術

Abstract

生物感測晶片是蛋白質體學中熱門發展的工具，利用微/奈米機電系統製程技術(Micro- and Nano-ElectroMechanical Systems)，可以方便製作出微型化可攜式元件，這些感測元件包括表面共振感測器、壓電晶體生物感測器、離子選擇性場效電晶體、電化學生物感測器等。然而要讓這些感測元件達到選擇性及專一性，蛋白質塗佈技術即為一個直接且有效的方式。蛋白質塗佈是將蛋白質或生物分子控制固定在特定空間，此方式是生物感測中一支基本但重要的技術。利用各種塗佈技術將生物分子定義於生物晶片上，再將分析液或待分析液與被塗佈的生物分子反應，以此方式可快速且大量分析未知的生物液及生物分子和化學物質間的交互作用。塗布的目標物可為蛋白質、DNA等各種生物分子。蛋白質塗佈可應用的範圍也極廣泛，包括基因體、癌症研究、藥物研發、臨床診斷、細胞研究、組織工程、生物分子鑑別等。 現有的蛋白質塗布技術中，很難同時達到高解析及可程式化的效果，同時也可能對蛋白質產生物理或化學性的傷害。本研究是利用微奈米機電系統製程技術，製作電控性的次微米級蛋白質塗佈元件，再利用螢光染色蛋白質配合螢光顯微鏡，分析塗佈結果，此方法不僅可以有效程式化蛋白質塗佈效果，產生高解析度塗佈式樣，也不會造成蛋白質的結構變形，以保持其活性，提高其應用之可行性。

Bio-sensor is one of the fundamental technologies for Proteomics. With the technology of the Micro- and Nano- Electromechanical Systems, it can be easily to manufacture the miniature portable device, that include the surface plasmon resonance detector, piezoelectric quartz crystal, ion-selective field effect transistor and eletrochemical bio-sensor. Protein patterning is a method which makes those devices more selective and sensitive. Protein patterning technology is to make proteins or bio-molecules be defined on some specific areas. Using some kinds of the patterning technologies to let the bio-molecules immobilized on a substrate, and then make the assays or reagents to be interact with the patterned bio-molecules, with this method it can be fleetly and enormously analysed the interact between the unknown lysate, bio-molecules and chemicals. The patterned object could be the proteins, DANs and all kinds of bio-molecules. The application of the protein patterning can be used widely, including the genome, cancer research, drug discovery, clinical diagnostics, cell studies, tissue engineering and bio-molecule identification. Nowadays, the protein patterning technologies are hard to achieve the high resolution and programmable patterns, moreover those methods might cause the patterned proteins physical or chemical damages. So this study is using the micro- and nano-electromechanical systems to manufacture the submicron electrically programmable protein patterning device, and utilizing the fluorescence labeling process and fluorescent microscope to analyse the patterned result. This method can not only make high resolution programmable patterns effectively without denaturing those proteins, but also sustain their activities to make sure the feasible of applications.