三口井除草劑毛細管電層析質譜儀分析與免擋板毛細管電層析質譜儀介面之開發

Abstract

本研究第一部份是以毛細管電層析質譜分離八種三口井除草劑。首先以等位沖堤的方式,尋找最佳的實驗參數,其參數包含緩衝溶液酸鹼值,操作電壓及有機修飾劑在緩衝溶液中的比例。在最佳條件下只能分出7根層析峰。為了達到更好的分離效果,我們參考文獻上有關於梯度沖堤的方法,設計出一個簡單的裝置。在梯度沖堤下,藉由連續地改變動相組成,可將八個除草劑解析開來。 有鑑於擋板(frits)在填充式毛細管電層析質譜造成的影響,實驗的第二部份是在毛細管兩端以拉尖縮口的方式取代擋板的燒製,可減少毛細管在製作及操作上的困難。並以低流速鞘流介面為游離化介面,發展出免擋板(fritless)毛細管電層析/電灑法質譜儀。

The eight triazines were studied by capillary electrochro matography/Mass Spectrometry(CEC/MS).Under isocratic elution condition including pH of the buffer solution, separation voltage and the ratio of organic solvent in the buffer solution. However , the eight triazines can not be separated under the optimized condition. In order to get better resolution, a simple device which can produce gradient elution is designed. Under gradient elution, the eight triazines could be separated. Frits have several drawbacks in packed-CEC/MS. The fabrication of frits by sintering the packing material in the two end of the packed-capillary is substituted by drawing out fine tapers. This approach eliminated the difficulties of the making frits and maked the operation of packed-CEC/MS easier than frit-CEC/MS. The performance of the fritless packed-CEC ESI (electrospray ionization)-MS was demostrated.