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標題: | 固態核磁共振光譜學於蛋白質二級結構之測量 Solid-State NMR Determination of Protein Secondary Structure |
作者: | Yun Mou 牟昀 |
指導教授: | 陳振中(Chun-Chung Chan) |
關鍵字: | 固態核磁共振,脈衝序列,蛋白質二級結構,扭轉角, solid-state NMR,pulse sequence,protein secondary structure,torsion angle, |
出版年 : | 2006 |
學位: | 碩士 |
摘要: | This work focused on developing solid-state NMR pulse sequence to determine the protein secondary structure. Chapter 1 gives a basic view of using solid-state NMR to obtain the structure information. The main thesis was discussed in four parts: Ch.2: R-TOBSY: isotropic polarization transfer in biological solids. Ch.3: MCSP: Multiple Chemical Shift Precession for selective polarization transfer. Ch.4: psi angle determination: using correlation between C' CSA and Ca−Ha tensors.
Ch.5: CONGEE: Correlation Of Non-Gamma Encoded Experiment. Each part is self-contained. The successful experiments of R-TOBSY (Chapter 2) and MCSP (Chapter3) in biological solids provide a crucial step for the psi angle determination in Chapter 4 and Chapter 5. Chapter 4~5 discussed the spin physics of the RACO experiment originally introduced by Ishii and co-workers in 1996. Through the analytical analysis, the optimal RACO experiment was designed and was employed for determination of the dihedral angles of L-alanine and DL-NAV. Brief conclusion and outlook were summarized in chapter 6. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32750 |
全文授權: | 有償授權 |
顯示於系所單位: | 化學系 |
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