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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32750完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳振中(Chun-Chung Chan) | |
| dc.contributor.author | Yun Mou | en |
| dc.contributor.author | 牟昀 | zh_TW |
| dc.date.accessioned | 2021-06-13T04:14:46Z | - |
| dc.date.available | 2006-07-26 | |
| dc.date.copyright | 2006-07-26 | |
| dc.date.issued | 2006 | |
| dc.date.submitted | 2006-07-24 | |
| dc.identifier.citation | 1. Mou, Y.; Chan, J. C. C., Frequency selective polarization transfer based on multiple chemical shift precession. Chem. Phys. Lett. 2006, 419, (1-3), 144-148.
2. Mou, Y.; Chao, J. C. H.; Chan, J. C. C., Efficient spin-spin scalar coupling mediated C-13 homonuclear polarization transfer in biological solids without proton decoupling. Solid State Nucl. Magn. Reson. 2006, 29, (4), 278-282. 3. Tseng, Y. H.; Mou, Y.; Mou, C. Y.; Chan, J. C. C., Double-quantum NMR spectroscopy based on finite pulse RFDR. Solid State Nucl. Magn. Reson. 2005, 27, 266-270. 4. Lin, K. S. K.; Tseng, Y. H.; Mou, Y.; Hsu, Y. C.; Yang, C. M.; Chan, J. C. C., Mechanistic Study of Apatite Formation on Bioactive Glass Surface Using 31P Solid-State NMR Spectroscopy. Chem. Mater. 2005, 17, 4493-4501. 5. Huang, S. J.; Tseng, Y. H.; Mou, Y.; Lin, S. B.; Huang, S. H.; Lin, C. P.; Chan J.C.C., Spectral editing based on selective excitation and Lee-Goldburg cross-polarization under magic angle spinning. Solid State Nucl. Magn. Reson. 2006, 29, (4), 272-277. 6. Wang, X. G.; Chen, C. C.; Chen, S. Y.; Mou, Y.; Cheng, S. F., Arenesulfonic acid functionalized mesoporous silica as a novel cid catalyst for the liquid phase Beckmann rearrangement of cyclohexanone oxime to epsilon-caprolactam. APPLIED CATALYSIS A-GENERAL 2005, 281, (1-2), 28147-54. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32750 | - |
| dc.description.abstract | This work focused on developing solid-state NMR pulse sequence to determine the protein secondary structure. Chapter 1 gives a basic view of using solid-state NMR to obtain the structure information. The main thesis was discussed in four parts: Ch.2: R-TOBSY: isotropic polarization transfer in biological solids. Ch.3: MCSP: Multiple Chemical Shift Precession for selective polarization transfer. Ch.4: psi angle determination: using correlation between C' CSA and Ca−Ha tensors.
Ch.5: CONGEE: Correlation Of Non-Gamma Encoded Experiment. Each part is self-contained. The successful experiments of R-TOBSY (Chapter 2) and MCSP (Chapter3) in biological solids provide a crucial step for the psi angle determination in Chapter 4 and Chapter 5. Chapter 4~5 discussed the spin physics of the RACO experiment originally introduced by Ishii and co-workers in 1996. Through the analytical analysis, the optimal RACO experiment was designed and was employed for determination of the dihedral angles of L-alanine and DL-NAV. Brief conclusion and outlook were summarized in chapter 6. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T04:14:46Z (GMT). No. of bitstreams: 1 ntu-95-R93223001-1.pdf: 2113273 bytes, checksum: 3985d2709bc8435c4a89eebe247b2787 (MD5) Previous issue date: 2006 | en |
| dc.description.tableofcontents | Chapter 1 Structure Determination by Solid-State NMR 1
1.1 Solid-State NMR versus Solution-State NMR 1 1.2 Pulse Sequence in Static Solid-State NMR 2 1.3 Magic Angle Spinning (MAS) 3 1.4 Pulse Sequence under MAS 4 1.5 Structure Information Determined by SSNMR 4 1.5.1 Chemical-shift based conformation determination 4 1.5.2 Distance constraints 5 1.5.3 Angular constraints 5 1.6 Summary 9 Chapter 2 R-TOBSY without proton decoupling 13 2.1 Introduction 13 2.2 Brief Review of TOBSY-Like Sequences 14 2.3 Simulations on No Decoupling R-TOBSY 16 2.4 Experimental 19 2.4.1 Sample preparation 19 2.4.2 Solid-state NMR 19 2.5 Results and Discussion 20 2.6 Conclusion 24 Chapter 3 Selective Polarization Transfer Based on Multiple Chemical Shift Precession 28 3.1 Introduction 28 3.2 Theory 29 3.2.1 Two-spin system 29 3.2.2 Three-spin system 32 3.3 Experimental 32 3.4 Results and Discussion 34 Chapter 4 Psi Angle Determination by RACO 42 4.1 Introduction 42 4.2 Theory 43 4.2.1 Correlation analysis 44 4.2.2 Numerical simulations 46 4.2.3 General consideration 47 4.2.4 Sensitivity to Psi angle 52 4.2.5 Experimental consideration 53 4.3 Experimental 54 4.3.1 Sample preparation 54 4.3.2 NMR experiments 54 4.3.3 Numerical simulations 55 4.3.4 Data process 56 4.4 Results 56 4.4.1 1D spectra 56 4.4.2 2D spectra 56 Chapter 5 Correlations of Non-Gamma Encoded Experiments 62 5.1 Introduction 62 5.2 Correlation Analysis 63 5.3 Rotor-phase Encoded Experiments 68 5.4 Magnetization-Phase Encoded Experiments 76 5.5 Discussion of Rotor-Phase Encoded and Magnetization Encoded Experiments 79 5.6 Supercycle-Phase Encoded Experiments 82 Chapter 6 Conclusions and Outlook 88 | |
| dc.language.iso | en | |
| dc.title | 固態核磁共振光譜學於蛋白質二級結構之測量 | zh_TW |
| dc.title | Solid-State NMR Determination of Protein Secondary Structure | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 94-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 黃太煌(Tai-Huang Huang),陸駿逸(Chun-Yi Lu) | |
| dc.subject.keyword | 固態核磁共振,脈衝序列,蛋白質二級結構,扭轉角, | zh_TW |
| dc.subject.keyword | solid-state NMR,pulse sequence,protein secondary structure,torsion angle, | en |
| dc.relation.page | 89 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2006-07-25 | |
| dc.contributor.author-college | 理學院 | zh_TW |
| dc.contributor.author-dept | 化學研究所 | zh_TW |
| 顯示於系所單位： | 化學系 | |
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