利用酸前處理提高纖維酵素水解蔗渣效率之研究

Abstract

本研究以蔗渣為原料，探討利用稀硫酸與稀鹽酸，於不同溫度（30、60、90℃），不同酸濃度（0.25、0.2、0.15M）與不同前處理時間（20、40、60分鐘）等條件下進行前處理，先分析前處理後之水解液含還原糖之濃度，再就殘留之固體以纖維水解脢，於pH值4.6、溫度50℃、酵素液濃度21U/mL、基質比1%等條件下進行酵素水解，探討前處理溫度、時間與酸濃度對於酵素水解轉換率之影響。研究結果發現：在前處理溫度90℃下，水解液中之還原醣濃度隨著酸濃度與前處理時間之增加而增加。經過0.25M之硫酸，於前處理溫度90℃、前處理時間60分鐘等條件下前處理者，其後續之酵素水解效果最好，其總轉換率可達到50%。 關鍵字：乙醇、酸處理、蔗渣、酵素水解。

Dilute sulfuric acid and hydrochloric acid pretreatment of bagasse before enzymatic hydrolysis of cellulose was investigated in this study. The biomass at a solid loading rate of 5% was pretreated at different temperature (30, 60, 90℃) with different acid concentrations (0.2, 0.15, 0.1, 0.05M) and residence times (20, 40, 60 min). Reducing sugars and total sugars in the prehydrolyzate were analyzed. In addition, the solid residues were hydrolyzed by cellulase from Trichoderma reesei C2730 to investigate the enzymatic digestibility. While bagasse was pretreated at 90℃ with the increasing acid concentration and residence time, the amount of reducing sugar in the filtrates increasing. Maximun yields were obtained when the pretreatment step was performed at 90℃ for 60 min with 0.20M H2SO4.