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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 謝志誠 | |
| dc.contributor.author | Po-Shen Chou | en |
| dc.contributor.author | 周柏伸 | zh_TW |
| dc.date.accessioned | 2021-06-13T03:39:20Z | - |
| dc.date.available | 2009-08-30 | |
| dc.date.copyright | 2006-08-30 | |
| dc.date.issued | 2006 | |
| dc.date.submitted | 2006-07-25 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32258 | - |
| dc.description.abstract | 本研究以蔗渣為原料，探討利用稀硫酸與稀鹽酸，於不同溫度（30、60、90℃），不同酸濃度（0.25、0.2、0.15M）與不同前處理時間（20、40、60分鐘）等條件下進行前處理，先分析前處理後之水解液含還原糖之濃度，再就殘留之固體以纖維水解脢，於pH值4.6、溫度50℃、酵素液濃度21U/mL、基質比1%等條件下進行酵素水解，探討前處理溫度、時間與酸濃度對於酵素水解轉換率之影響。研究結果發現：在前處理溫度90℃下，水解液中之還原醣濃度隨著酸濃度與前處理時間之增加而增加。經過0.25M之硫酸，於前處理溫度90℃、前處理時間60分鐘等條件下前處理者，其後續之酵素水解效果最好，其總轉換率可達到50%。
關鍵字：乙醇、酸處理、蔗渣、酵素水解。 | zh_TW |
| dc.description.abstract | Dilute sulfuric acid and hydrochloric acid pretreatment of bagasse before enzymatic hydrolysis of cellulose was investigated in this study. The biomass at a solid loading rate of 5% was pretreated at different temperature (30, 60, 90℃) with different acid concentrations (0.2, 0.15, 0.1, 0.05M) and residence times (20, 40, 60 min). Reducing sugars and total sugars in the prehydrolyzate were analyzed. In addition, the solid residues were hydrolyzed by cellulase from Trichoderma reesei C2730 to investigate the enzymatic digestibility. While bagasse was pretreated at 90℃ with the increasing acid concentration and residence time, the amount of reducing sugar in the filtrates increasing. Maximun yields were obtained when the pretreatment step was performed at 90℃ for 60 min with 0.20M H2SO4. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T03:39:20Z (GMT). No. of bitstreams: 1 ntu-95-R93631011-1.pdf: 1251405 bytes, checksum: b06cb044b286f1b56f0f1fec04c5cc83 (MD5) Previous issue date: 2006 | en |
| dc.description.tableofcontents | 誌謝 I
摘要 II ABSTRACT III 目錄 IV 圖目錄 VII 表目錄 XI 第一章 前言 1 第二章 文獻探討 5 2-1 木質纖維素 5 2-2 生質乙醇製程 10 2-2-1稀酸水解製程 10 2-2-2濃酸水解製程 12 2-2-3 酵素水解製程 13 2-3前處理 16 2-3-1機器粉碎 17 2-3-2裂解 17 2-3-3 蒸氣爆裂法 18 2-3-4 高壓熱水處理法 18 2-3-5 稀釋酸處理法 20 2-3-6 稀釋酸穿流處理法 20 2-3-7 石灰前處理法 21 2-3-8 氨水前處理法 21 2-3-9 二氧化碳爆裂法 22 2-4酵素水解 23 2-4-1 纖維水解脢 23 2-4-2 纖維水解脢之製作 24 2-4-3 纖維水解脢之活性 25 2-4-4 纖維水解脢作用機制 27 2-4-5 影響酵素水解之因素 27 2-5醱酵 30 2-6 同步醣化與醱酵 32 2-7蔗渣之應用 37 第三章 研究方法 38 3-1實驗材料與設備 38 3-2分析項目及方法 42 3-2-1蔗渣之組成 42 3-2-2標準曲線之建立與水解液之分析 43 3-3實驗方法 44 3-3-1前處理 44 3-3-2酵素水解 45 第四章 結果與討論 47 4-1蔗渣成分測定 47 4-2標準曲線 48 4-3稀酸前處理 49 4-3-1前處理溫度之影響 49 4-3-2前處理酸濃度之影響 52 4-3-3小結 53 4-4酵素水解 55 4-4-1酵素水解條件分析 55 4-4-1-1水解時間之影響 55 4-4-1-2 基質比之影響 57 4-4-2前處理後之酵素水解 59 4-4-2-1前處理時間之影響 59 4-4-2-2前處理酸濃度之影響 64 4-4-2-3前處理溫度之影響 68 4-4-3 比較 71 第五章 結論 73 參考文獻 74 | |
| dc.language.iso | zh-TW | |
| dc.title | 利用酸前處理提高纖維酵素水解蔗渣效率之研究 | zh_TW |
| dc.title | Improving Enzymatic Hydrolysis of Bagasse
by Acid Pretreatment | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 94-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳世銘,周楚洋,李允中 | |
| dc.subject.keyword | 乙醇,酸處理,蔗渣,酵素水解, | zh_TW |
| dc.subject.keyword | ethanol,acid pretreatment,bagasse,enzymatic hydrolysis, | en |
| dc.relation.page | 83 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2006-07-27 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 生物產業機電工程學研究所 | zh_TW |
| 顯示於系所單位： | 生物機電工程學系 | |
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