Sumoylation在調控Ets-1中所扮演的角色

Abstract

Ets-1 has been demonstrated to play some roles in the regulation of T lymphocytes, which including T cell development, differentiation and apoptosis. Recently, Ets-1 has been reported to be a modulator of T helper cell cytokine secreting profiles which include interleukin-2, interleukin-4, interleukin-10 and interferon-gamma. Although the regulation of Ets-1 has been extensively studied, the role of sumoylation on the function of Ets-1 remains elusive. Here, we demonstrated that abolishing the consensus SUMO conjugation motif in Ets-1 did not alter the transcription of IFN-gamma by Ets-1 and T-bet. Furthermore, we demonstrated that the transactivation ability of Ets-1 on the reporter carrying Ets-1-binding region had been no changed when we mutated the sumoylation sites. Moreover, wild-type and sumoylated Ets-1 have the similar transactivation ability in the presence of PIAS proteins, SUMO E3 ligase. These results imply that sumoylation may play no role on Ets-1’s transcriptional activities. However, since the sumoylation of Ets-1 is real, the functions of sumoylated Ets-1 need to be further discovered .