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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 繆希椿 | |
| dc.contributor.author | Hsiao-Wei Tsao | en |
| dc.contributor.author | 曹孝瑋 | zh_TW |
| dc.date.accessioned | 2021-06-13T03:37:23Z | - |
| dc.date.available | 2006-08-04 | |
| dc.date.copyright | 2006-08-04 | |
| dc.date.issued | 2006 | |
| dc.date.submitted | 2006-07-26 | |
| dc.identifier.citation | Barton, K., N. Muthusamy, et al. (1998). 'The Ets-1 transcription factor is required for the development of natural killer cells in mice.' Immunity 9(4): 555-63.
Bories, J. C., D. M. Willerford, et al. (1995). 'Increased T-cell apoptosis and terminal B-cell differentiation induced by inactivation of the Ets-1 proto-oncogene.' Nature 377(6550): 635-8. Dittmer, J. (2003). 'The biology of the Ets1 proto-oncogene.' Mol Cancer 2(1): 29. Ghysdael, J., A. Gegonne, et al. (1986). 'Identification and preferential expression in thymic and bursal lymphocytes of a c-ets oncogene-encoded Mr 54,000 cytoplasmic protein.' Proc Natl Acad Sci U S A 83(6): 1714-8. Grenningloh, R., B. Y. Kang, et al. (2005). 'Ets-1, a functional cofactor of T-bet, is essential for Th1 inflammatory responses.' J Exp Med 201(4): 615-26. Hahn, S. L., B. Wasylyk, et al. (1997). 'Modulation of ETS-1 transcriptional activity by huUBC9, a ubiquitin-conjugating enzyme.' Oncogene 15(12): 1489-95. Hart, A. H., R. Reventar, et al. (2000). 'Genetic analysis of ETS genes in C. elegans.' Oncogene 19(55): 6400-8. Hsu, T. and R. A. Schulz (2000). 'Sequence and functional properties of Ets genes in the model organism Drosophila.' Oncogene 19(55): 6409-16. Johnson, E. S. (2004). 'Protein modification by SUMO.' Annu Rev Biochem 73: 355-82. Kingsley, D. M., N. A. Jenkins, et al. (1989). 'A molecular genetic linkage map of mouse chromosome 9 with regional localizations for the Gsta, T3g, Ets-1 and Ldlr loci.' Genetics 123(1): 165-72. Kola, I., S. Brookes, et al. (1993). 'The Ets1 transcription factor is widely expressed during murine embryo development and is associated with mesodermal cells involved in morphogenetic processes such as organ formation.' Proc Natl Acad Sci U S A 90(16): 7588-92. Lavender, P., D. Cousins, et al. (2000). 'Regulation of Th2 cytokine gene transcription.' Chem Immunol 78: 16-29. Li-Weber, M. and P. H. Krammer (2003). 'Regulation of IL4 gene expression by T cells and therapeutic perspectives.' Nat Rev Immunol 3(7): 534-43. Macauley, M. S., W. J. Errington, et al. (2006). 'Beads-on-a-string, characterization of ETS-1 sumoylated within its flexible N-terminal sequence.' J Biol Chem 281(7): 4164-72. Mackereth, C. D., M. Scharpf, et al. (2004). 'Diversity in structure and function of the Ets family PNT domains.' J Mol Biol 342(4): 1249-64. Mullen, A. C., F. A. High, et al. (2001). 'Role of T-bet in commitment of TH1 cells before IL-12-dependent selection.' Science 292(5523): 1907-10. Nunn, M. F., P. H. Seeburg, et al. (1983). 'Tripartite structure of the avian erythroblastosis virus E26 transforming gene.' Nature 306(5941): 391-5. Obika, S., S. Y. Reddy, et al. (2003). 'Sequence specific DNA binding of Ets-1 transcription factor: molecular dynamics study on the Ets domain--DNA complexes.' J Mol Biol 331(2): 345-59. Oda, N., M. Abe, et al. (1999). 'ETS-1 converts endothelial cells to the angiogenic phenotype by inducing the expression of matrix metalloproteinases and integrin beta3.' J Cell Physiol 178(2): 121-32. Ouyang, W., M. Lohning, et al. (2000). 'Stat6-independent GATA-3 autoactivation directs IL-4-independent Th2 development and commitment.' Immunity 12(1): 27-37. Prigge, J. R. and E. E. Schmidt (2006). 'Interaction of protein inhibitor of activated STAT (PIAS) proteins with the TATA-binding protein, TBP.' J Biol Chem 281(18): 12260-9. Reddy, S. Y., S. Obika, et al. (2003). 'Conformations and dynamics of Ets-1 ETS domain-DNA complexes.' Proc Natl Acad Sci U S A 100(26): 15475-80. Savage, P. D., C. A. Hanson, et al. (1987). 'Identification of a restriction fragment length polymorphism involving the oncogene ETS-1 on chromosome 11q23.' Blood 70(1): 327-9. Seeler, J. S. and A. Dejean (2003). 'Nuclear and unclear functions of SUMO.' Nat Rev Mol Cell Biol 4(9): 690-9. Sementchenko, V. I. and D. K. Watson (2000). 'Ets target genes: past, present and future.' Oncogene 19(55): 6533-48. Skalicky, J. J., L. W. Donaldson, et al. (1996). 'Structural coupling of the inhibitory regions flanking the ETS domain of murine Ets-1.' Protein Sci 5(2): 296-309. Teruyama, K., M. Abe, et al. (2001). 'Role of transcription factor Ets-1 in the apoptosis of human vascular endothelial cells.' J Cell Physiol 188(2): 243-52. Watson, D. K., M. J. McWilliams-Smith, et al. (1986). 'Conserved chromosomal positions of dual domains of the ets protooncogene in cats, mice, and humans.' Proc Natl Acad Sci U S A 83(6): 1792-6. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32220 | - |
| dc.description.abstract | Ets-1 has been demonstrated to play some roles in the regulation of T lymphocytes, which including T cell development, differentiation and apoptosis. Recently, Ets-1 has been reported to be a modulator of T helper cell cytokine secreting profiles which include interleukin-2, interleukin-4, interleukin-10 and interferon-gamma. Although the regulation of Ets-1 has been extensively studied, the role of sumoylation on the function of Ets-1 remains elusive. Here, we demonstrated that abolishing the consensus SUMO conjugation motif in Ets-1 did not alter the transcription of IFN-gamma by Ets-1 and T-bet. Furthermore, we demonstrated that the transactivation ability of Ets-1 on the reporter carrying Ets-1-binding region had been no changed when we mutated the sumoylation sites. Moreover, wild-type and sumoylated Ets-1 have the similar transactivation ability in the presence of PIAS proteins, SUMO E3 ligase. These results imply that sumoylation may play no role on Ets-1’s transcriptional activities. However, since the sumoylation of Ets-1 is real, the functions of sumoylated Ets-1 need to be further discovered . | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T03:37:23Z (GMT). No. of bitstreams: 1 ntu-95-R93449001-1.pdf: 355543 bytes, checksum: 610bbd5f82a8318dd52289a9c4ee1e92 (MD5) Previous issue date: 2006 | en |
| dc.description.tableofcontents | Acknowledgement i
摘要 ii Abstract iii CHAPTER I Introduction 1 1.1 The role of T helper cells in immunity 1 1.1.1 T helper cell development and differentiation 1 1.2 An overview of Ets-1 transcription factor 2 1.2.1 The discovery of Ets-1 2 1.2.2 Protein structure of Ets-1 2 1.2.3 Biological roles of Ets-1 4 1.2.4 Regulation of Ets-1 5 1.3 An overview of sumoylation 5 1.3.1 SUMO: small ubiquitin-like modifier 5 1.3.2 The SUMO conjugation pathway 6 1.3.3 Biological functions of SUMO 7 1.4 Rationale to study the relationship between Ets-1 and sumoylation in T helper cells 7 CHAPTER II Material and Methods 9 2.1 Experimental procedures 9 2.1.1Constructions 9 2.1.2 Sumoylation sites prediction 11 2.1.3 Site-directed mutagenesis 12 2.1.4 Cell culture 13 2.1.5 HEKHEK293T cells transfection 13 2.1.6 Luciferase assay 13 2.1.7 Purification of GST-Ets1 fusion protein for the production of Ets-1 polyclonal antibodies 14 2.1.8 Co-immunoprecipitation 15 2.2 Experimental materials 16 2.2.1 Enzymes 16 2.2.2 Kits 17 2.2.3 Chemicals, reagents and materials 17 2.2.4 Instruments and softwares 19 2.2.5 Media, solutions and buffers 20 CHAPTER III Results 25 3.1 The functional synergy between T-bet and wild-type or sumoylation sites mutated Ets-1 is comparable 25 3.2 The role of PIAS proteins in IFNγ transactivation by T-bet 25 3.3 The Ets-1 minimal reporter luciferase assay system 26 3.4 The transactivation abilities of wild type or sumoylation sites mutated Ets-1 27 3.5 The transcriptional activities of wild type Ets-1 in the presence of PIAS proteins. 27 3.6 The transcriptional activities of Ets-1 mutants in the presence of PIAS family proteins. 28 CHAPTER IV Discussions 29 REFERENCES 33 FIGURES AND TABLES 36 | |
| dc.language.iso | en | |
| dc.subject | 細胞激素 | zh_TW |
| dc.subject | 輔助型T細胞 | zh_TW |
| dc.subject | Ets-1 | en |
| dc.subject | SUMO | en |
| dc.title | Sumoylation在調控Ets-1中所扮演的角色 | zh_TW |
| dc.title | The Role of Sumoylation on the Regulation of Ets-1 | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 94-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 司徒惠康,林國儀 | |
| dc.subject.keyword | 細胞激素,輔助型T細胞, | zh_TW |
| dc.subject.keyword | Ets-1,SUMO, | en |
| dc.relation.page | 41 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2006-07-27 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 免疫學研究所 | zh_TW |
| 顯示於系所單位： | 免疫學研究所 | |
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