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Effect of Product containing Pine Bark Extract on Lipid Metabolism in Hamsters
|Publication Year :||2006|
|Abstract:||法國海濱松（ Pinus maritima）樹皮萃取物，商品名為Pycnogenol®（PYC），已有很多文獻指出具有多項生理療效，唯其脂質代謝部份較為缺乏。本研究之目的在於探討富含法國海濱松樹皮萃取物產品對於倉鼠脂質代謝之影響，並藉此推測其降血脂的機制。本實驗以AIN-93M飼料為基本飼料組成，配製高脂高膽固醇飼料（額外添加豬油5%與膽固醇添加0.2%），正對照物質則使用β-穀固醇（β-sitosterol，添加量為4%），處理組則分別額外添加0.2%、0.4%、0.8%三個劑量的富含松樹皮萃取物產品粉末。實驗動物48隻倉鼠以體重隨機分組，分別為正常組、高脂組、正對照組，以及三個處理組（分別為低、中、高劑量）共六組，餵食標準AIN-93M飼料馴養2週，之後除正常組之外，餵食修飾AIN-93M高脂高膽固醇飼料8週誘發高血脂症（hyperlipidemia）。實驗期間結束後倉鼠禁食12小時，犧牲取血，肝臟、副腎脂、副睪脂則秤重後冷凍保存。結果顯示，三個處理組與高脂組相比，在血清TC（total cholesterol）均有顯著下降的效果，但三者之間並無顯著差異；血清TG（triglyceride）方面，處理組之中隨產品添加濃度增加有線性下降的效果，但在低劑量組並無顯著差異；血清中LDL膽固醇（low-density lipoprotein cholesterol）的濃度隨產品添加量的升高呈線性降低；但血清中HDL膽固醇（high-density lipoprotein cholesterol）方面，處理組與高脂組均無顯著差異。肝臟脂質方面，處理組肝中TC在高劑量組與高脂組相比有顯著下降的現象，肝中TG則以低劑量組降低的效果最好，而試驗物質對於肝臟脂肪酸的影響並不明顯。在排除脂質的影響方面，糞便中TC、TG、膽汁酸含量均隨添加劑量上升而降低。而在抗氧化能力方面，血清TBARS值在低、中劑量組均顯著低於高脂組，但高劑量組TBARS值顯著高於高脂組，三個處理組隨產品添加比例提高，其血清TBARS值呈劑量關係上升；肝臟TBARS則隨添加松樹皮萃取物增多呈線性下降的趨勢，在高劑量組與高脂組相比有顯著的降低；且三個處理組其血清LDL氧化的遲滯時間（lag time）均較高脂組要長，高劑量組在氧化測定時間之內均尚未完全氧化。|
The trade name of French marine pine bark extract is Pycnogenol ®（PYC）, and many researches have referred that PYC possesses some physiological activities, but that is poor on hypolipidemic. The aim of this study is to investigate the effect of French marine pine ( Pinus maritima ) bark extract on lipid metabolism, and from above to presume the mechanism of the hypolipidemic activity. In this experiment, 48 hamsters were divided into 6 groups according to their weight, and gave them the modified diet according to AIN-93M. The high-fat diet contained 5% lard and 0.2% cholesterol additionally, whereas the positive-control contained 4% β-sitosterol. The three experimental groups contained 0.2%, 0.4%, 0.8% ( low, medium, and high dose ) product powder rich of PYC, additionally. In the results, serum-TC ( total cholesterol ) was significantly decreased compared with high-fat group, but there were no differences between the three experimental groups, and serum-TG ( triglyceride ) was decreased in linear manner, but not significant in low dose group. The serum-LDL ( low density lipoprotein cholesterol ) was also significantly decreased in linear manner, but PYC did not influence the concentration of serum-HDL ( high density lipoprotein cholesterol ). In liver lipid, liver-TC was significantly decreased in high dose group, whereas liver-TG was decreased most significantly in low dose group, but the consistence percentage of liver-fatty acid was not influenced. On the effect of excreting lipid, feces-TC, feces-TG, and feces-bile acid were all decreased in dose dependent fit. Serum-TBARS was decreased significantly compared with high-fat group in low and medium dose group, but increased significantly in high dose group, and the serum-TBARS in three experimental groups were increasing in linear manner. Liver-TBARS in experimental groups decreased, and significant in high dose group. Also, the lag time of LDL-C oxidation was prolonged in experimental groups significantly, and the LDL-C was not completely oxidized in high dose group during detection time.
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