探討由人類及Epstein-Barr病毒所譯成的microRNAs於鼻咽自然殺手/T細胞淋巴癌中之表現模式

Abstract

鼻咽自然殺手/T細胞淋巴癌是一種特殊類型的結節外淋巴瘤，與EB病毒有很強的關連性。MicroRNAs(miRNAs)會影響到多種癌症的發生及生物學上功能，然而，鼻咽自然殺手/T細胞淋巴癌上miRNAs的分布及表現量還是未知數。因此我們在鼻咽自然殺手/T細胞淋巴癌病人檢體中篩選55隻人類miRNAs及39隻EB病毒miRNAs的表現模式。我們由46個鼻咽自然殺手/T細胞淋巴癌病人的組織石蠟包埋蠟塊中純化出total RNA，以即時定量聚合酶連鎖反應來分析定量人類及EB病毒 miRNAs的表現情況。我們分別使用三種統計方法來作分析，由T Test結果可知，EBV-miR BART13*和Has miR-155、miR-210及miR-320的表現於早期和晚期的鼻咽自然殺手/T細胞淋巴癌中有顯著性差異，和第二次重複的結果相同，兩次結果都可分析出有顯著性差異。由集群分析結果可知，我們無法利用miRNAs的表現模式區分出鼻咽自然殺手/T細胞淋巴癌早期和晚期階段。

Nasal Natural Killer/T-Cell Lymphoma (NNKTL) is a distinctive type of extranodal lymphoma with a strong association with Epstein-Barr virus (EBV). MicroRNAs have been implicated in the development and biological behaviors of a variety of cancers. However, expression patterns of miRNAs in NNKTLs are still unknown. We screened the expression patterns of 39 EBV-encoded and 55 human microRNAs in NNKTL. Total RNAs were isolated from paraffin-embedded tissues of NNKTLs. Real-time quantitative PCR was used to analyze the expression levels of EBV and human microRNAs. We used three statistical methods to analyze our QPCR results. From the T Test results, we can know that EBV-miR BART13 * and Hsa miR-155, miR-210 and miR320 have significant difference between the early and late stage in NNKTL and from the repeated data also have same result. From the Cluster analysis results, we could not separate the early and late stage in NNKTL clearly base on miRNAs expression patterns.