幾丁聚醣/雙官能基酸交聯水膠於皮膚創傷敷料

Abstract

幾丁聚醣(chitosan)為極具發展潛力的生醫材料，近年來已被廣泛應用於骨骼組織修復、藥物釋放系統以及創傷敷料等方面。幾丁聚醣其具有良好的生物性質，例如生物相容性、生物降解性以及抗菌性，且幾丁聚醣對於血液有促進凝結止血的效果。然而幾丁聚醣的低機械性質和快降解速率均限制其應用範圍，特別在具有溶菌

Chitosan is a potential material for biomedical applications. Recently, it is widely applied in bone tissue regeneration, drug delivery systems and wound dressings. Chitosan has outstanding biological properties, including biocompatible, biodegradable and antibacterial ability. Moreover, chitosan has hemostatic ability for promoting blood to coagulate together. However, the applications of chitosan are limited for its low mechanical properties and rapid degradation trend, especially in the environment containing lysozyme. Chitosan existing in acid environment becomes a cationic polyelectrolyte due to its amine group, it can easily form polyelectrolyte complexes with other anionic polyelectrolytes and growth factors, or form crosslink structure with carboxylic group by composing amide bond. Through this strategy, the properties of chitosan can be modified and enhanced. In this research, in order to enhance the mechanical properties and to ease the biodegradation rate, the glutamic acid, succinic acid and succinic anhydride are applied to form crosslink structure with chitosan separately, with the repeating unit ratio of 80:20 to 50:50. Besides, this study investigates physicochemical characterization and biological properties of the crosslinked hydrogels. Via the implant study, animal test was conducted to evaluate the possibility that crosslinked hydrogels could be a potential wound dressing material. Results of wound closure and histological tissue observation showed that chitosan/diacid crosslinked hydrogels could accelerate the process of wound healing and tissue regeneration. The study reveals chitosan could suppress the infiltration of inflammatory cells and accelerate fibroblast proliferation while the diaicds could enhance epithelial migration. Thus, chitosan/diacid crosslinked hydrogels could be potential materials for wound dressing applications.