發展脂肪幹細胞片於幾丁聚醣-明膠薄膜並應用於傷口癒合

Abstract

In this study, we used a composite biomaterial composed of adipose-derived stem cells (ADSC) and gelatin/chitosn film (C/G film) with ascorbic acid 2-phosphate A2P to promote the wound healing process. The C / G film was prepared from chitosan and gelatin in 0.4% AcOH at a ratio of 3 to 7, and was dried at room temperature, 37, and 60 ° C. And then, the dried C / G films were neutralized by 0.1, 0.5, and 1N NaOH. The production of C/G film at 37℃ and neutralized by 1N NaOH can get the optimum condition.

The ADSCs were cultured on the C / G film for 5 days to form the cell sheet, and the gelatin from C/G film was released by 74.6% (measured by bicinchoninic acid assay).The expression of type 1 collagen from the ADSCs on C/G film was more than on TCPS at 7 day (measured by western blot). The animal experiment shows that the wound of B6 mice covered with cell sheet can not be speeded up the healing, but reduces the formation of granulation tissue obviously. However, there was no significant difference in the Lanyu pig model.