梨形鞭毛蟲拓樸異構酶Ⅲalpha對於囊體壁基因的表現之影響

Abstract

梨形鞭毛蟲屬於人畜共通的單細胞真核寄生蟲，造成的疾病為giardiasis，他會造成腹瀉、腹痛，嚴重時可能會導致營養吸收不良的情況，梨形鞭毛蟲主要分成兩個型態-滋養體和囊體，在宿主體內的時候主要是以滋養體的型態寄生，也是造成giardiasis病徵的原因，當梨形鞭毛蟲經由宿主腸道排出體外後會以囊體的型態在外界生存，不管是在滋養體的雙核或是囊體的四核，對於有關於梨形鞭毛蟲的遺傳物質的任何機轉都是需要精細且準確地進行，當遺傳物質DNA在進行像是複製、轉錄、重組、染色體的濃縮或分離時會造成拓樸學結構上的問題，因此會需要特殊的蛋白質-拓樸異構酶來幫助遺傳物質解決結構上問題。我們經由序列的分析比對找出梨形鞭毛蟲中屬於topoisomerase type IA的topoisomerase Ⅲalpha(gTOP3

Giardiasis is a common disease caused by Giardia lamblia. The symptoms of giardiasis included diarrhoea, abdominal pain, weight loss, and malabsorption. Giardia has two major types: trophzoite and cyst. In trophzoite form, it’s can parasitize in the host’s instestinal epithelium and cause giardiasis. Cysts form of G. lamblia are passed in the faeces and protect Giardia against the environment. Both trophozoite with two nuclei or cyst with four tetraploid nuclei all need to proceed accuratent mechanism for DNA. When DNA is carrying out replication, transcription, recombination, chromosome compaction and segregation, some topological problems would occur these processes would result some topological problems. Therefore, topoisomerases can be applied to solve these construction problems to ensure DNA is normal. We have analyzed the G. lamblia genome and found topoisomerase Ⅲalpha(gTOP3