脂肪幹細胞療法合併羊膜共同培養系統對牙周骨質再生之效益

Abstract

牙周病是發生於牙周組織之慢性發炎疾病，嚴重的牙周病可能會造成牙齒缺損，影響日常生活飲食、發音等生理狀態甚鉅，然而臨床上仍無完全治癒之治療方式，目前研究期望運用組織工程與幹細胞療法在缺失部位協助牙周骨質新生。脂肪幹細胞於疾病治療的研究上已有許多成功的經驗，並證實其具有多能性分化能力，利用脂肪幹細胞合併羊膜片共同培養，希望利用羊膜具有抗發炎、抗血管新生、降低感染風險，且有豐富的生長因子之特性，將此共同培養系統運用於大鼠模式的牙周骨缺損組織上，研究結果顯示脂肪幹細胞合併羊膜片共同培養系統可促進牙周骨缺損修復之能力，成功建立牙周骨質缺損研究之動物模式，並提供脂肪幹細胞與羊膜片移植應用於牙周病臨床治療之參考。

Periodontal disease is a chronic inflammatory reaction of periodontal tissues that potentially leads to tooth loss. It can affect daily activity, such as chewing and speech. Unfortunately, there is no effective therapy for periodontal disease. Use of tissue engineering technologies and stem cell therapy on Periodontal bone regeneration is a very promising treatment. Adipose-derived stem cell has been proved to its multipotency and have significant outcome in many disorders. Amniotic membrane is anti-inflammation, anti-angiogenic, low immunogenicity, and enriched with growth factor. We create a co-culture system with adipose-derived stem cell and amniotic membrane in periodontal osseous defect of rat model. The result suggest that the co-culture system could enhance periodontal bone regeneration. We believed this study could provide the base of the co-culture system as stem cell therapy and amniotic membrane transplantation in clinical periodontology.