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  3. 化學工程學系
Please use this identifier to cite or link to this item: http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/16663
Title: 以柴油培養之Rhodococcus erythropolis NTU-1結塊處理水包油(O/W)型乳化液
De-emulsification of oil-in-water emulsion by Rhodococcus erythropolis NTU-1 bioflocculi grown in diesel
Authors: Yueh-Ting Lai
賴岳廷
Advisor: 劉懷勝
Keyword: Rhodococcus erythropolis NTU-1,破乳化,油相回收百分比,細胞表面疏水性,水包油(O/W)型乳化液,
Rhodococcus erythropolis NTU-1,de-emulsification,oil recovery,cell surface hydrophobicity,oil-in-water emulsion,
Publication Year : 2014
Degree: 碩士
Abstract: NTU-1是從石油汙泥中分離並透過16S rDNA序列鑑定確立其所屬菌屬為Rhodococcus erythropolis。經由培養、烘乾後,可得到NTU-1結塊之粉末,此粉末具有很好的破乳化能力。
本研究比較以柴油培養的NTU-1粉末及以正十六烷培養的NTU-1粉末之破乳化效率。經實驗結果發現,以柴油培養的NTU-1粉末具有生產成本較低、使用量較少以及破乳化能力較佳之優勢。若以柴油培養的NTU-1粉末進行破乳化測試,其最適添加量與Tween-80體積存在著三分之二次方的關係。於O/W/T比例為4/1/0.01,在480分鐘時油相回收百分比可達95%。此外,當乳化液的油/水比(O/W)較大時或連續相黏度較低時,可提升油相回收百分比。細胞表面疏水性是影響破乳化重要的因素。實驗結果顯示,微生物細胞疏水性與油相回收百分比呈現正相關。
綜括上述,以烘乾後的NTU-1粉末處理水包油(O/W)型乳化液具有相當的潛力以及發展性。
NTU-1, a highly efficient demulsifying strain, was isolated from petroleum contaminated soil and identified as Rhodococcus erythropolis by 16S rDNA gene analysis. NTU-1 powder with high demulsifying performance could be obtained by heat-drying after collecting bioflocculi in medium.
This study first evaluated the demulsifying performance of NTU-1 cultured by diesel and n-hexadecane, and found that NTU-1 powder by diesel is a better choice for its lower cost and better performance. At an optimal quantity of dried NTU-1 powder for de-emulsification that was proportional to the volume of Tween-80 to the 2/3 power, a recovery of 95 % oil was achieved in 480 min. This was made possible when the oil/water/Tween80 volume ratio was set at 4/1/0.01. The higher O/W ratio and the lower viscosity both contributed to such a high oil recovery rate.
The positive correlation between CSH (MATH) and oil recovery ratio also implies that the diesel grown NTU-1 culture may provide a commercial scale-up method to break oil -in-water emulsion directly at the primary oil recovery site.
URI: http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/16663
Fulltext Rights: 未授權
Appears in Collections:化學工程學系

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