雷射誘導石墨烯修飾玻璃碳電極於乙醯氨基酚檢測之研究

Abstract

近年來，乙醯氨基酚（Acetaminophen, APAP）在臨床診斷之研究日益增多。本研究旨在開發一種快速、靈敏且具成本效益的電化學感測元件，目標為偵測液體中之APAP。本感測器以CO₂雷射誘導聚醯亞胺（Polyimide, PI）薄膜生成石墨烯（Laser-induced graphene, LIG），再修飾於玻璃碳電極（Glassy carbon electrode, GCE）表面，形成 LIG/GCE 感測面。材料特性透過四點探針（Four-point probe）、場發射掃描式電子顯微鏡（Field emission scanning electron microscope, FESEM）、拉曼光譜儀（Raman spectrometer）、X 光光電子能譜儀（X-ray photoelectron spectroscopy, XPS）與X射線繞射（X-ray diffractometer, XRD）進行驗證，以評估其微觀結構、化學組成與結晶性。在電化學性能分析方面，本研究採用循環伏安法（Cyclic voltammetry, CV）與方波伏安法（Square wave voltammetry, SWV）進行測試。結果顯示，LIG/GCE 靈敏度（Sensitivity）為0.157 µA·µM⁻¹·cm⁻²、檢測極限（Limit of detection, LOD）為3.95 µM，寬廣的線性範圍（Linear range）5–120 µM，並展現出優異的抗干擾能力與選擇性。本研究展示 LIG 技術在感測APAP領域的潛力，具備快速製備與低成本等優勢，可望應用於人類尿液中對APAP的偵測。

In recent years, acetaminophen (APAP) has attracted growing attention in clinical diagnostics monitoring. This study aims to develop a rapid, sensitive, and cost-effective electrochemical sensor for APAP detection in aqueous media. The sensor uses laser-induced graphene (LIG), fabricated by CO₂ laser irradiation of a polyimide (PI) film, to modify a glassy carbon electrode (GCE), forming a LIG/GCE sensing platform. The structural and chemical properties of LIG were characterized via Four-point probe, FESEM, Raman spectroscopy, XPS, and XRD. Electrochemical performance was evaluated using cyclic voltammetry and square wave voltammetry. Results show the LIG/GCE electrode exhibits a sensitivity of 0.157 µA·µM⁻¹·cm⁻²,a limit of detection (LOD) of 3.95 µM, and a broad linear range of 5–120 µM. The sensor demonstrates excellent selectivity and anti-interference capability, making it suitable for complex matrices. This study demonstrates the potential of LIG technology for APAP sensing, offering advantages such as rapid fabrication and low cost, and shows promise for application in detecting APAP in human urine.