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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 謝淑貞 | zh_TW |
| dc.contributor.advisor | Shu-Chen Hsieh | en |
| dc.contributor.author | 魏灩蘋 | zh_TW |
| dc.contributor.author | Yen-Pin Wei | en |
| dc.date.accessioned | 2025-02-21T16:25:03Z | - |
| dc.date.available | 2025-02-22 | - |
| dc.date.copyright | 2025-02-21 | - |
| dc.date.issued | 2024 | - |
| dc.date.submitted | 2024-12-19 | - |
| dc.identifier.citation | 1. 衛生福利部國民健康署, 中華民國 110 年癌症登記報告. 2023.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/96757 | - |
| dc.description.abstract | 根據衛生福利部民國110年癌症登記報告，大腸癌在台灣的發病率位居第二，死亡率則排名第三。當大腸癌轉移到其他器官時，患者的五年生存率急劇下降。研究指出，某些癌細胞在遷移過程中不會完全經歷上皮-間質轉化 （EMT），而是同時保留部分上皮和間質特徵，以集體遷移為主要方式遷移方式。集體遷移雖需要更多能量，但也增強了癌細胞的轉移能力。中國橄欖萃取物（COE）已知能干擾癌細胞能量代謝並抑制其生長。本研究旨在探討COE是否通過影響集體遷移來抑制癌細胞的遷移能力。結果顯示，COE在一般細胞培養環境下能有效抑制癌細胞的生長和遷移，而對EMT相關蛋白的表現無顯著影響。但其影響E-cadherin在細胞內的分佈，可能從而改變遷移邊緣細胞的形態。此外，在不同環境條件下實驗結果顯示，環境中的丙酮酸含量會影響COE對癌細胞生存率的抑制效果，而環境中的血清濃度則影響其對癌細胞遷移的抑制。這一差異可能源於細胞生存與遷移所需能量規模的不同。然而，透過本研究結果顯示環境中丙酮酸的含量（pyruvate）雖對COE引起的遷移邊緣細胞形態變化有影響，但並不改變COE抑制癌細胞遷移的效果。總結來看，在本研究模型中，COE 本身所造成的能量缺乏已足以抑制癌細胞遷移，丙酮酸所造成的能量變化無法干擾此一現象，但遷移邊緣細胞形態變化是否為COE抑制癌細胞遷移的重要因子仍需更進一步確認。 | zh_TW |
| dc.description.abstract | According to the 2021 Cancer Registry Annual Report by Taiwan's Ministry of Health and Welfare, colorectal cancer ranks second in incidence and third in mortality among all cancers in Taiwan. When colorectal cancer metastasizes to other organs, the five-year survival rate of patients markedly declines. Studies indicate that some type of cancer cells do not fully undergo epithelial-to-mesenchymal transition （EMT） during migration, but retain both epithelial and mesenchymal characteristics, primarily migrating in clusters. Although collective migration requires more energy, it also enhances the metastatic capability of cancer cells. Chinese olive extract （COE） is known to interfere with cancer cell energy metabolism and inhibit their growth. This study aims to explored whether COE inhibits cancer cell migration by affecting collective migration. The results showed that COE effectively inhibited cancer cell survival growth and migration in normal cell culture conditions without significantly affecting the expression of EMT-related proteins. However, it influences the distribution of E-cadherin within cells, potentially altering the morphology of cells at the migration edge. Additionally, the experimental results under different environmental conditions revealed that the pyruvate content in the environment affected COE's ability to inhibit cancer cell survival, while serum concentration influenced its ability to inhibit cancer cell migration. This difference may be due to the varying energy demands required for cell survival and migration. Nevertheless, the study results show that although the environmental pyruvate content affects COE-induced morphological changes of cells at the migration edge, it did not alter COE's inhibitory effect on cancer cell migration. In conclusion, in this study model, the energy deficiency caused by COE alone is sufficient to inhibit cancer cell migration, and the energy changes induced by pyruvate cannot disrupt this phenomenon. However, further investigation is needed to confirm whether the morphological changes in migration edge cells are a key factor in COE’s inhibition of cancer cell migration. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2025-02-21T16:25:03Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2025-02-21T16:25:03Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 摘要 I
Abstract II 目次 IV 圖次 VII 縮寫表 VIII 第一章 文獻回顧 1 第一節 大腸直腸癌 1 1. 大腸直腸癌的流行病學 1 2. 大腸直腸癌的致病機制 1 3. 大腸直腸癌的轉移 2 4. 大腸直腸癌的治療 2 第二節 癌細胞轉移相關機制 4 1. 癌細胞轉移 4 2. 癌細胞的代謝調控 6 第三節 中國橄欖 10 1. 中國橄欖的成分分析 10 2. 中國橄欖的生理活性 10 第二章 研究目標與實驗架構 13 第一節 研究目標 13 第二節 實驗架構 14 第三章 材料與方法 15 第一節 實驗材料 15 1. 中國橄欖水萃物殘渣甲醇萃取乙酸乙酯層萃取物 （COE） 15 2. 細胞培養 16 3. 實驗藥品 17 4. 實驗耗材 18 5. 抗體 19 6. 商業套組 22 7. 儀器設備 23 8. 數據處理軟體 24 第二節 實驗方法 25 1. 細胞培養 25 2. 細胞存活率實驗 28 3. 細胞遷移試驗（2-well insert） 29 4. 群落生成試驗（clonogenic assay） 30 5. 細胞總蛋白質萃取 31 6. BCA 蛋白定量法 32 7. 十二烷基硫酸鈉聚丙烯酰胺凝膠電泳（SDS-PAGE） 33 8. 西方點墨法（Western blot） 35 9. 細胞總 RNA 萃取 37 10. 反轉錄 PCR（reverse transcription PCR, RT-PCR） 38 11. 即時聚合酶鏈反應（Real-time PCR, qPCR） 39 12. 免疫螢光染色（Immunofluorescence stain, IF stain） 40 第四章 實驗結果 42 第一節 COE 對於HCT 116的生長及遷移的影響 42 1. COE 對於HCT 116 生存率的影響 42 2. COE 對於 HCT 116 增殖的影響 43 3. COE 抑制 HCT 116 遷移的能力 43 第二節 COE 影響 leader cell 和 follower cell 的型態 45 第三節 在不同環境下 COE 對於 HCT 116的生長及遷移的影響 47 1. 環境對於 COE 抑制 HCT 116 生存的影響 47 2. 環境對於 COE 抑制 HCT 116 遷移能力的影響 48 第四節 Pyruvate 對於COE抑制 HCT 116 細胞遷移的影響 50 第五節 COE 對於 HCT116 遷移的抑制與脂質代謝的關聯 52 第五章 討論 53 第一節 常見細胞培養環境下COE 對於HCT116 的效力確認 53 第二節 不同的環境條件下改變COE 對於HCT116 的效力影響 54 第三節 COE 抑制 HCT116 集體遷移可能的機制探討 56 1. COE對HCT116細胞遷移模式的影響不依賴於EMT相關蛋白表現的改變 56 2. 透過影響能量 57 3. 其他可能的機制 58 第六章 結論 59 第七章 圖 60 第八章 參考文獻 90 第九章 附錄 96 | - |
| dc.language.iso | zh_TW | - |
| dc.subject | 中國橄欖 | zh_TW |
| dc.subject | 大腸癌 | zh_TW |
| dc.subject | 集體遷移 | zh_TW |
| dc.subject | EMT | zh_TW |
| dc.subject | 癌細胞代謝 | zh_TW |
| dc.subject | Cancer metabolism | en |
| dc.subject | Colon cancer | en |
| dc.subject | Chinese olive | en |
| dc.subject | Collective migration | en |
| dc.subject | EMT | en |
| dc.title | 探討中國橄欖萃取物於不同培養條件下造成大腸癌細胞生長及集體遷移狀態的影響 | zh_TW |
| dc.title | The Effects of Chinese Olive (Canarium album L.) Extract on Colorectal Cancer Cell Growth and Collective Migration Under Different Culture Conditions | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 113-1 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 蘇純立;廖憶純;劉瑋文 | zh_TW |
| dc.contributor.oralexamcommittee | Chun-Li Su;Yi-Chun Liao;Wei-Wen Liu | en |
| dc.subject.keyword | 大腸癌,中國橄欖,集體遷移,EMT,癌細胞代謝, | zh_TW |
| dc.subject.keyword | Colon cancer,Chinese olive,Collective migration,EMT,Cancer metabolism, | en |
| dc.relation.page | 99 | - |
| dc.identifier.doi | 10.6342/NTU202404733 | - |
| dc.rights.note | 未授權 | - |
| dc.date.accepted | 2024-12-20 | - |
| dc.contributor.author-college | 生物資源暨農學院 | - |
| dc.contributor.author-dept | 食品科技研究所 | - |
| dc.date.embargo-lift | N/A | - |
| 顯示於系所單位： | 食品科技研究所 | |
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